American Journal of Heterocyclic Chemistry 2016; 2(1): 8-12 http://www.sciencepublishinggroup.com/j/ajhc doi: 10.11648/j.ajhc.20160201.12 Spectroscopic Identification and Synthesis of Derivatives of Pyrimidine 2- Thione Via Two Different Spacers Moayed J. Mohammed 1 , Ahmed Kh. Ahmed 1 , Faris T. Abachi 2, * 1 Department of Chemistry, College of Education for pure Science, University of Mosul, Mosul, Iraq 2 Department of Pharmaceutical Chemistry, College of Pharmacy, University of Mosul, Mosul, Iraq Email address: farisabachi@yahoo.com (F. T. Abachi) * Corresponding author To cite this article: Moayed J. Mohammed, Ahmed Kh. Ahmed, Faris T. Abachi. Spectroscopic Identification and Synthesis of Derivatives of Pyrimidine 2- Thione Via Two Different Spacers. American Journal of Heterocyclic Chemistry. Vol. 2, No. 1, 2016, pp. 8-12. doi: 10.11648/j.ajhc.20160201.12 Received: October 28, 2016; Accepted: November 12, 2016; Published: December 10, 2016 Abstract: A new series of pyrimidine -2-thione derivatives were synthesized via two routes and designated as antifolate. The heterocyclic ring was prepared by direct reaction of benzoyl acetone with thiosemicarbiazide in acidic medium. Also, the pyrimidine-2-thione ring reacts with p-aminobenzoic acid to form 4-amino-N-(4-methyl-6-phenyl)-2-thioxypyrimidine 1[2H] – yl benzamide. The second route synthesis 5-[{4-methyl-6-phenyl-2-thioxo pyridine-1-yl amino}-4-(4-methyl phenylsulfonamido)-5-oxobenzoyl] pentanoic acid. All structures were elucidate by their physical & spectroscopic data FTIR, 1 H & 13 C NMR. Keywords: Antifolate, P-aminobenzoic Acid, Pyrimidine, NMR 1. Introduction In recent years, 5- substituted pyrimidine-2-thione derivatives have drawn great attention for their anti cancer activity by inhibiting DNA synthesis. [1, 2]. Pyrimidine and their derivatives have been found to possess a broad spectrum of biological activities such as antimicrobial, anti-inflammatory, analgesic, antiviral and anticancer activities [3]. Nitrogen containing heterocyclic ring such as pyrimidine is a promising structural moiety for drug design. Pyrimidine derivatives form a component in a number of useful drugs and are associated with many biological and therapeutically activities [4, 5]. The aim of this study was designated to synthesis of pyrimidine -2-thione derivatives via two different spacers as expected as antifolate agent. 2. Experimental 2.1. Chemicals All reagents were purchased from commercial sources and used without further purification, the employed chemicals and there supplier from BDH &Fluka companies. 2.2. General All melting points were uncorrected and determined by the Electro-thermal IA 9100 melting point apparatus. All reactions were monitored by TLC using pre-coated Aluminum sheet silica gel Merck 60 F 254 and were visualized by iodine vapourand detected the spots using UV lamp and purification using micro column with silica gel. The infra-red (IR) spectra were recorded using potassium bromide disc technique on Bruker optics Co.; Alpha P, IR Spectrophotometer. The proton nuclear magnetic resonance ( 1 H NMR) & Carbon -13 nuclear resonance ( 13 C NMR) spectra were performed on Bruker 400MHz Spectrophotometer using tetramethylsilane (TMS) as internal standard. Chemical shift values (δ) are given using parts per million scale (ppm) in UK. Chemical naming, calculation of molecular weight (M. wt) of new compounds were performed by Chem Draw 12 software. 2.3. Route A 2.3.1. 1-Amino-4-Methyl–6–Phenyl Pyrimidine 2-Thione [6] Mix 0.01 mole of benzoyl acetone with 0.01 mole