A5.76 Poster Session — Monday 29th June 2009 Effects of different source and level of protein in feed mixtures on liver and intestine histology of the common carp (Cyprinus carpio, Linnaeus, 1758) Bozidar Raskovic (University of Belgrade, Faculty of Agriculture), Marko Stankovic (University of Belgrade, Faculty of Agriculture), Zorka Dulic (University of Belgrade, Faculty of Agriculture), Zoran Markovic (University of Belgrade, Faculty of Agriculture), Nada Lakic (University of Belgrade, Faculty of Agriculture), Vesna Poleksic (University of Belgrade, Faculty of Agriculture) In order to investigate effects of different feed mixtures in extruded feed for carp two 90 days feeding experiments on common carp yearlings (Cyprinus carpio, Linnaeus, 1758) were carried out. Effects on digestive tract morphology were studied. The study was performed in the Laboratory for fish nutrition at the Faculty of Agriculture, University of Belgrade. In the first experiment diets with different levels of fishmeal and soybean meal were used. In the second experiment the level of proteins in fishmeal varied. Distal intestine and liver of experimental carp were sampled at the start and after 90 days of the experiment. In both experiments no major pathological changes were recorded, therefore we used some morphometric parameters for the estimation of histological status of two organs which are usually most affected by different diets — liver and the intestines. In liver, average nuclear area was measured and compared between fish fed different diets. In the intestines, quantification of enterocytes height, and length of intestinal folds was performed. The results of histological and morphometrical analysis are discussed in relation with feed composition. This study suggests that histological and morphometric analyses can be useful additional tools when feed efficiency is being investigated in fish. Email Address for correspondence: raskovic@agrif.bg.ac.rs doi:10.1016/j.cbpa.2009.04.163 A5.77 Poster Session — Monday 29th June 2009 Development of enrichment criteria for zebrafish(Danio rerio) used in laboratory studies Luanne Wilkes (University of Exeter), L. Leaver (University of Exeter), Kath Sloman (University of Plymouth), G. Readman (AstraZeneca), T. Williams (AstraZeneca), S. Owen (AstraZeneca), Rod Wilson (Uni- versity of Exeter) At present there is little available information concerning fish welfare within laboratory studies, beyond that concerning the environ- mental conditions required for their survival. As such there is currently no legislation regarding provision of any forms of environmental enrichment such as is commonly recommended for mammals and birds. To study the possible benefits of enrichment for juvenile zebrafish (Danio rerio), we kept two groups of fish in either “enriched” or “barren” tanks. Those classified as enriched contained 3-D glass rod structures which fish could swim between and around in order to provide them both with a more complex environment and a refuge from conspecifics and human observers. During the study, behavioural data relating to fish activity levels, shoaling density and use of enrichment, was collected and analysed. Results of this data seem to suggest that the enrichment provided has no discernible effect on either activity levels or shoaling density of fish. Importantly, fish did not spend a significantly greater or lesser amount of time in areas containing enrichment than those without. Following termination of the study, fish whole bodies are being analysed for both cortisol and ion levels to determine if provision of enrichment has any effect on these commonly used physiological indicators of stress. In addition to this, to account for any possible effects of ontological change on the benefits of need for enrichment, we are now repeating the study using the mature siblings of the original fish tested. Email Address for correspondence: w261@exeter.ac.uk doi:10.1016/j.cbpa.2009.04.164 A5.78 Poster Session — Monday 29th June 2009 Effects of dispersed crude oil upon the cardiovascular physiology and the metabolic scope of juvenile Golden Mullet Liza Aurata Thomas Milinkovitch (Littoral Environnement Sociétés (LIENSs), UMR 6250 CNRS, University of La Rochelle), C. Le François (Littoral Environnement Sociétés (LIENSs), UMR 6250 CNRS, University of La Rochelle), J. Lucas (Littoral Environnement Sociétés (LIENSs), UMR 6250 CNRS, University of La Rochelle), Hélène Thomas Guyon (Littoral Environnement Sociétés (LIENSs), UMR 6250 CNRS, University of La Rochelle), S. LeFloch (Cedre), N. Imbert (Littoral Environnement Sociétés (LIENSs), UMR 6250 CNRS, University of La Rochelle) Dispersants used in case of oil spills aim at transferring oil from the water surface into the water column. Consequently, when dispersants are applied before the oil slick reaches the coastline, the hydrocarbons bioavailability increases for aquatic organisms living in water-column or on sea floor. Our study aimed at assessing the effects of the oil dispersion in Liza aurata, a species living in coastal areas. During 48 h, fish were exposed to different conditions which could occur during an oil spill: to the dispersants itself, to the water soluble fraction of oil (WSF), to a chemically dispersed crude oil and to a mechanically dispersed crude oil. In addition, a control condition was tested. Hydrocarbons concentra- tions were analysed all along the exposure period (on both their soluble and particular fraction). After 48 h-exposure to one of these treatments, some key physiological functions were assessed in L. aurata. At the heart level, myocardium performances were evaluated, through measure- ments of the contractility of ventricular muscle strips. The efficiency of the adrenergic pathway and the calcium handling capacity by the sarcoplasmic reticulum were tested using adrenaline (1 nM and 1 μM) and ryanodine (10 μM). On the other hand, both aerobic metabolic scope and critical swimming speed were measured in fish challenged through a swimming-step protocol. In case of oil spills, dispersion-related impairments of these functions will be discussed in term of the animal fitness. Email Address for correspondence: thomas.milinkovitch@hotmail.fr doi:10.1016/j.cbpa.2009.04.165 A5.79 Poster Session — Monday 29th June 2009 Ultrastructural study of the pallial reproductive tract of the protandric neogastropod Coralliophila meyendorffii (Gastropoda: Muricidae) Ma Jose Amor Perez (Universitat de Barcelona), Ma José Amor Perez (Universitat de Barcelona), Montserrat Ramón (Institut de Ciencies del Mar (CSIC), Barcelona), Mercè Durfort (Universitat de Barcelona) Abstracts / Comparative Biochemistry and Physiology, Part A 153 (2009) S90–S113 S112