Area Under Curve Method Development for Etodolac in Bulk and Tablet dosage form Vaibhav G Bhamare 1* , Renuka R Joshi 2 , RD Amrutkar 3 1 Department of Research Scholar, Bhupal Nobles University, Udaipur, India; 2 Department of Pharmaceutical Quality Assurance, Mahatma Gandhi Vidyamandir’s Pharmacy College, Panchavati, Nashik, India; 3 Department of Pharmaceutical Chemistry, MGV’s SPH College of Pharmacy, Malegaon, India ABSTRACT A simple,precise, rapid and economical spectroscopic method has been developed for the determination of Etodolac in bulk and tablet dosage form. In methanol Etodolac shows absorbance maxima at 281nm. Method used was area under curve in which area incorporated in the wavelength range of 275nm-285nm.Etodolac obeys Beer’s law in concentration range 0-60µg/ml. Calibration curves were plotted at selected wavelengths and it was found to be linear (r2=0.9997) Linearity for detector response was observed in the concentration range of 5-30µg/ml. The recovery studies established accuracy of the proposed method and results validated according to ICH guideline. Precision and Accuracy studies were carried out and results were satisfactory. Robustness of the given method was studied by using two wavelength, 279nm and 283nm respectively. Results were found satisfactory and reproducible. The developed method was successfully applied to estimate the amount of Etodolac in pharmaceutical formulation. Keywords: Area under curve; accuracy, precision; spectroscopic method. INTRODUCTION Etodolac is non steroidalanti inflammatory drug which is belongs to pyranocarboxylic acid class developed in the 1970s. It blocks production of certain natural substances that causes inflammation [1]. Etodolac is chemically 1, 8-Diethyl-1, 3, 4, 9- tetrahydropyrone (3, 4-b) indole -1-acetic acid. Molecular formula of Etodolac is C17H21No3 [2].Cox present in two separate entities, one is Cox-1 and other is Cox-2[3, 4]. It inhibits synthesis of peripheral prostaglandins, by decreasing the activity of the Cox enzyme. Cox-1 protect the integrity of the stomach lining and sustain normal renal function in a kidney .Cox-2 plays a vital role in both control of cell growth and inflammation [5].Peak serum concentration achieved within 2 hours of oral administration of Etodolac 200mg and 400mg respectively [6] It is rapidly metabolized in the liver, followed by renal elimination as the primary route of excretion [7]. It is used for rheumatoid arthritis and osteoarthritis, postoperative pain and inflammation [8].It is used as anti- inflammatory agent, analgesic antipyretic and cyclooxygenase inhibitor. Etodolac is official in the United States Pharmacopoeia and British Pharmacopoeia [9]. Method development is the process of verifying that an analytical method is good enough for exercise to measure the concentration of an API in a particular compounded dosage form which allow basicmeasures to be employed to confirm that an analysis procedure, precisely and consistently will deliver a trustworthy measurement of an active ingredient in a compounded preparation [10].Primary purpose of method development to generate date regarding efficiency, safety, impurity, stability (that shows degradation of product), bioavailability and the effect of manufacturing parameter (that shows steadiness of the product) [11]. MATERIALS AND METHODS The spectrophotometric analysis was carried out using a Labindia UV-3000 Uv/Vis spectrophotometer with 1 cm matched quartz cell. The spectra were obtained with the instrumental parameters as follows:Wavelength range (nm): J o u r n a l of D e v e lo p i n g D r u g s ISSN: 2329-6631 Journal of Developing Drugs Research Article *Corresponding author: Vaibhav G Bhamare, Department of Research Scholar, Bhupal Nobles University, Udaipur, India, Tel: 7588176846; E-mail: vaibhav.bhamre@gmail.com Received date: April 02, 2021; Accepted date:April 16, 2021; Published date: April 23, 2021 Citation: Bhamarre VG, Joshi RR, Amrutkar RD (2021) AreaUnder Curve Method Development for Etodolac in Bulk and Tablet dosage form. J Develop Drugs. 10:207. Copyright: ©2021 Bhamare VG, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. J Develop Drugs, Vol.10 Iss.3 No:1000207 1