Research article Volume 12 Issue 4 - January 2021 DOI: 10.19080/OAJS.2021.12.555843 Open Access J Surg Copyright © All rights are reserved by Prof. Dr. Cassius Souza Physichemistry and Ionic Strength on Abiotic surfaces of Medical devices Influence in adherence and biofilm formation by Multi-Drug Resistant Corynebacterium Striatum Cassius Souza 1,2,4*# , Yuri Vieira Faria 1# , Higor Franceschi Motta 1 , Felipe de Oliveira Cabral 1 , Giorgio Silva- Santana 1 , Allan Motta Leal Pontes 1 , Darlan Ferreira de Souza 4 , Lincoln de Oliveira Sant’Anna 1 , Marcus Vinícius de Oliveira 3 , Louisy Sanches dos Santos 1 and Ana Luíza Mattos-Guaraldi 1 1 Department of Microbiology, Immunology and Parasitology, Rio de Janeiro State University, Brazil 2 Foundation Educational of the Ponds Region of Rio de Janeiro State, Brazil 3 Health Sciences Center, Institute of Microbiology Paulo de Góes, Federal University of Rio de Janeiro, Brazil 4 Augusto Motta University Center, Rio de Janeiro City # Cassius de Souza and Yuri Vieira Faria contributed equally for the first authorship in this manuscript Received: January 11, 2021; Published: January 29, 2021 *Corresponding author: Prof. Dr. Cassius de Souza, Laboratory of Diphtheria and Corynebacteriosis of Clinical Importance – LDCIC, Department of Microbiology, Immunology and Parasitology, Rio de Janeiro State University, Rio de Janeiro, Brazil. State University of Rio de Janeiro, Rio de Janeiro, Brazil; Foundation Educational of the Ponds Region of Rio de Janeiro State – Brazil. Yuri Faria Vieira, Departament of Microbiology, Imunology and Parasitology, Rio de Janeiro State University, Brazil Open Access J Surg 12(4): OAJS.MS.ID.555843 (2021) 001 Abstract Corynebacterium striatum is a Gram-positive bacillus and too potentially pathogenic microorganism with the ability to produce nosocomial outbreaks. Additionally, C. striatum has been associated with an increasing number of invasive infections as such as: sepsis, endocarditis, meningitis, osteomyelitis. However, there are a few studies focused on virulence factors that may contribute to elucidate the mechanisms concerned about healthcare associated infections by Corynebacterium spp. including C. striatum. The relevance of biofilm formation to development of nosocomial infections was recognized and the effects of antimicrobial agents on these surface-attached communities remain under investigation. Therefore, the biofilm formation by Corynebacterium striatum were validated quantitatively conform previous methodology. Additionally, o was analyzed by electron scanning microscopy of biofilm formation on abiotic substrates. Therefore, were used four different clones isolated in nosocomial outbreak in University Hospital in Rio de Janeiro city. The biofilm formation analysis was performed by CFU quantification and SEM according to previously described to the surface of glass and polyurethane, glass slides and catheters fragments were inoculated by immersion in 106 CFU. ml-1 bacterial suspension in Trypticase Soy Broth and incubated to 37ºC/48h. To quantitative evaluation, the formed biofilm was then extracted by abrasion and quantified by CFU count. To structural analysis, sections of glass coverslips and polyurethane catheters were fixed in 2.5% glutaraldehyde, post- fixed in 1% osmium tetroxide solution and dehydrated an ethanol gradient. Subsequently catheter segments were submitted to critical point drying with carbon dioxide, covered with 10nm gold layer, and examined with a JEOL JSM 5310 scanning electron microscope. The results revealed C. striatum ability to adhere to hydrophilic (glass) and hydrophobic polyurethane, abiotic surfaces at different intensities. Additionally, C. striatum strains showed biofilm formation in the polyurethane catheter surface 48h post-incubation and maturation of the biofilm resulting in the generation of a complex architecture with channels and pores that formed their three-dimensional structure the presence of extracellular matrix. Conclusion: All samples the of C. striatum tested adhere on substrates tested at different intensities and your complex structure has several characteristics that show the present of mature biofilm. Discussion: From these results, effective and appropriate measures should be taken to control this the hospital environment and thus to decrease the incidence of outbreaks caused by C. striatum. Keywords: Antimicrobial multidrug resistance; Bacteremia; Biofilm; Catheter-related infection; C. striatum; Nosocomial outbreak, Surgical wards. Abbreviations: CFU: colony-forming unit; CLSI: Clinical da Laboratory Standards Institute; CVC: central venous catheter; HMJ: Hospital Menino Jesus; HUPE: Hospital Universitário Pedro Ernesto; ICU: intensive care unit; MDR: multidrug-resistant; MDS: multidrug-susceptible; MIC: minimum inhibitory concentration; MLSB: macrolides, lincosamides and streptogramins B; PFGE: pulsed-field gel electrophoresis; QRDR: quinolone-resistance determinant region; UERJ: Universidade do Estado do Rio de Janeiro; UPGMA: unweighted-pair group method using average linkages. Introduction Corynebacterium spp. is widely disseminated in the environment and can colonize the skin and mucous membranes of humans as part of the normal microbiota [1]. Because of these characteristics, as well as challenges in its identification, Corynebacterium spp. remain frequently considered as contaminants in clinical microbiological laboratories and by health