_____________________________________________________________________________________________________ *Corresponding author: Email: Olanike.alajo@elizadeuniversity.edu.ng; International Journal of Pathogen Research 10(4): 18-30, 2022; Article no.IJPR.89495 ISSN: 2582-3876 Occurrence, Physicochemical Properties and Antibiotic Resistance Patterns of Enteric Bacteria Isolated from Well Water in Ilara-Mokin, Ondo State, Nigeria Funmilayo Olanike Alajo a,b* and Felix Akinsola Akinyosoye b a Department of Microbiology, Elizade University, P.M.B. 002, Ilara-Mokin, Nigeria. b Department of Microbiology, School of Life Sciences, Federal University of Technology, P.M.B. 704, Akure, Nigeria. Authors’ contributions This work was carried out in collaboration between both authors. Author FAA designed the study. Author FOA managed the literature searches, conducted the experiment, performed the statistical analysis, wrote the protocol and wrote the first draft of the manuscript. Authors FAA and FOA managed the analyses of the study. Both authors read and approved the final manuscript. Article Information DOI: 10.9734/IJPR/2022/v10i430257 Open Peer Review History: This journal follows the Advanced Open Peer Review policy. Identity of the Reviewers, Editor(s) and additional Reviewers, peer review comments, different versions of the manuscript, comments of the editors, etc are available here: https://www.sdiarticle5.com/review-history/89495 Received 12 May 2022 Accepted 18 July 2022 Published 29 August 2022 ABSTRACT Aims: TheoCcurrence and antibiotic resistance patterns of enteric bacteria isolated from well water sources and their physicochemical profile was ascertained in this study. Molecular analysis of the bacterial isolates from well water samples was conducted via polymerase chain reaction (PCR)- based identification. Study Design: Experimental design. Methodology: A total of 12 wells were collected in triplicates from groundwater sources (wells), and mean values were obtained. The first batch of samples were collected during the dry season in January and February, 2020. The second batch of samples were collected during the rainy season in July 2020. The detection of coliforms and other enteric bacteria were conducted via the most probable number (MPN) method. Polymerase chain reaction (PCR)-based identification and characterization of bacterial isolates were employed. Antibiotic susceptibility test was done using the Kirby Bauer disc diffusion technique. The determination of the physicochemical properties of the well water samples were conducted following customary protocol. Original Research Article