Abstract / Toxicology Letters 280S (2017) S166–S169 S167 If a registrant is required to perform an OECD 443 study, but has already performed an OECD 421 or 422 Reproduc- tive/Developmental Toxicity Screening test, an additional Dose Range Finding study may or may not be recommended & this will depend upon the design and outcome of the screening test, with recommendations bespoke for each chemical. The OECD443 study design may also influence the choice of strain of rat to be used. If the full study (including F1 Cohorts 1, 2 and 3) is required a strain of rat with a large litter size and consis- tent sex ratio is advised. A standard ECHA study design (F1 Cohort 1 only) could utilise a wider range of commonly used rat strains, including those that elicit a smaller litter size or more variable sex ratio. Since some of the ages at which evaluations are required on the OECD 443 study are different to those on routine reproductive toxicology studies, it is vital that investigations are adequately sup- ported by Historical Control Data. Envigo will perform an historical control data study in both Sprague Dawley & Han Wistar rats to ensure adequate support. http://dx.doi.org/10.1016/j.toxlet.2017.07.465 P-04-11-04 Effects of parathion on the activity of mitochondrial glutamate dehydrogenase from rat pancreatic Langerhans islets Hamid Reza Jamshidi 1 , Elham Ebrahimi 2 , Ali Moradi 2 1 Toxicology, Yazd Shahid Sadoughi University of Medical Sciences, Yazd, Islamic Republic of Iran 2 Toxicology, Pharmacy School, Yazd Shahid Sadoughi University of Medical Sciences, Yazd, Islamic Republic of Iran The main propose of the present study was to determine the effects of parathion on the activity of glutamate dehydrogenase (GDH) as the key enzyme of Langerhans islet for secretion of insulin. Parathion was administered intraperitoneally at doses of 0.5, 1, 1.5 and 2 mg/kg. Langerhans islets were isolated from the pancreas of rats by a standard collagenase digestion, separation by centrifuga- tion, and hand-picking technique. The activity of the mitochondrial GDH was determined in the islets homogenates. Parathion at tested doses (0.5, 1) had no effect on GDH secretion but at doses of 1.5 and 2 mg/kg significantly (p < 0.05) increased production of glutamate after 4 days treatment. It is concluded that GDH is a component of parathion-induced changes in release of improper insulin. It might be said parathion can increase production of glutamate that increase secretion of insulin but this insulin is not enough to over- come glucose production from liver that affected by parathion. http://dx.doi.org/10.1016/j.toxlet.2017.07.466 P-04-11-05 Endocrine disruption and carcinogenesis: Evaluation of neoplasms in endocrine organs of rat in Carbendazim carcinogenic chronic study Viktoriia Lisovska, Nadiya Nedopytanska, Eugene Bagley, Olena Reshavska L.I. Medved’s Research Center of Preventive Toxicology, Food and Chemical Safety, Ministry of Health, Ukraine, Kyiv, Ukraine US EPA has classified Carbendazim as a possible carcinogen to humans. Carbendazim carcinogenicity may be determine its aneu- genic and endocrine disruption properties. The aim is to analyze the rat tumors of the endocrine organs in carcinogenic study. In the present study 560 rats, Wistar (280/sex/group) were used. Carbendazim by gavage at 0, 5, 25, 75 mg/kg/b.w./day for 104 weeks. Carbendazim 98% carcinogenicity study carry out according to OECD451. There are 214 tumors recognize in experiment. Common tumor sites were thyroid (62), mammary (41), pituitary (31), Hard- erian (1), adrenal glands (10); testis (16), brain (1), lung (3), esophagus (1), liver (8), pancreas (1), uterus (4) ovary (5), haem- atopoietic system (19), soft tissue (7), skin (3). The benign tumor rate is exceed the malignant. Tumors of endocrine organs were more often. Histopathological types of tumors: thyroid follicular adenoma, carcinoma; adrenal phaeochromocytoma, cortical ade- noma, ganglioneuroma, carcinoma; pituitary adenoma, carcinoma; Leydigoma; mammary adenoma, fibroadenoma; uterus adeno- carcinoma; ovary fibroma, cystadenoma, thecoma, granulose cell tumor. The tumor rate (according to the effective number) in male was 14; 21; 10 contrary to 9 in the control group; in female – 37; 32; 30 to 17, respectively. The incidence of pituitary tumors were significantly increased in females treated with 75 mg/kg and in the mammary gland with 5 and 25 mg/kg. There were no differences between the groups in mean dose-depend in total tumor rate and in tumors of the endocrine system. Therefore, Carbendazim was shown to be epigenetic carcinogen as a potent endocrine disrupting substance. http://dx.doi.org/10.1016/j.toxlet.2017.07.467 P-04-11-06 The effect of MPA liver herbal supplementary on Type 2 diabetes Ali Akbar Malekirad 1,2 , Sahar Sadeghi khansari 3 , Habiballah Nazem 3 , Mohammad Abdollahi 1 1 Department of Toxicology, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran 2 Toxicology and Diseases Group, Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran 3 Department of Biology, Payame Noor University, Tehran, Islamic Republic of Iran Type 2 diabetes (T2D) is a chronic metabolic disorder that is known as a serious problem in the public health. It is characterized by high blood glucose level, insulin resistance, impaired lipid, and pro- tein and carbohydrate metabolism. Medicationercise and diet are considered as fundamental treatment protocols of T2D. Further- more, recently more attention has been paid to the beneficial effect of herbal medicine on T2D treatment. Hence the present study aimed to investigate the effect of MPA liver that insist of Melissa Officinalis, Cinnamomum zelanicum, Terminalia chebula, Cichorium intybus, Cynara scolymus and Silybum marianum on hypoglycaemic control. The study designed as a before-after clinical trial in 47 T2D patients. All participants were asked to drink the herbal tea mix- ture twice a day for 30 days. The biochemical parameters including fasting blood sugar (FBS), aspartate aminotransferase (ALT), ala- nine aminotransferase (AST), and alkaline phosphatase (ALP) were measured before and after intervention. The results represented a significant decrease in FBS (p < 0.05) while there were no significant changes in ALP, AST and ALT. In conclusion, the present outcomes suggest the herbal tea mixture including Melissa Officinalis, Cinnamomum zelanicum, Ter- minalia chebula, Cichorium intybus, Cynara scolymus and Silybum