How to Cite: Goel, K., Singh, R., Saini, V., & Bansal, S. (2022). Phytochemical estimation and evaluation of antioxidant activity of hydro-alcoholic and ethylacetate extracts of Tribulus terrestris linn. (gokhru) and Solanum nigrum linn (makoi). International Journal of Health Sciences, 6(S6), 7661–7676. https://doi.org/10.53730/ijhs.v6nS6.12158 International Journal of Health Sciences ISSN 2550-6978 E-ISSN 2550-696X © 2022. Manuscript submitted: 9 April 2022, Manuscript revised: 18 June 2022, Accepted for publication: 27 July 2022 7661 Phytochemical estimation and evaluation of antioxidant activity of hydro-alcoholic and ethylacetate extracts of Tribulus terrestris linn. (gokhru) and Solanum nigrum linn (makoi) Kirti Goel Research Scholar, Department of Pharmaceutical sciences, Maharishi Markandeshwar College of Pharmacy, MM (DU), Mullana, Ambala, Haryana, India Email: kirtijain2727@gmail.com Randhir Singh Associate Professor, Central University of Punjab, Bhatinda, Punjab, India Email: randhirsingh.dahiya@gmail.com Vipin Saini Director, RAAC, Maharishi Markandeshwar College of Pharmacy, MM (DU), Mullana, Ambala, Haryana, India Email: vipinsaini31@rediffmail.com Seema Bansal Professor, Department of Pharmacology, Maharishi Markandeshwar College of Pharmacy, MM (DU), Mullana, Ambala, Haryana, India *Corresponding author email: seema.bansal@mmumullana.org Abstract---Tribulus terrestris L. and Solanum nigrum L. has been used traditionally since ages. To explore the phytoconstituents (qualitatively and quantitatively) and estimate antioxidant activity of hydroalcoholic and ethyl acetate extracts of fruits of Tribulus terrestris L. and Solanum nigrum. Different methods were adopted for phytochemical screening. Evaluation of antioxidant potential was achieved by total anti-oxidant capacity assay, hydrogen peroxide scavenging activity, DPPH radical-scavenging activity, Nitric oxide radical scavenging activity and reducing power assay. The qualitative estimation of fruits of Tribulus terrestris L and of Solanum nigrum L. showed the presence of various phytoconstituents likewise tannins, saponins, flavonoids and terpenoids. Quantitative estimation revealed the presence of flavonoids (5.01%w/w), saponins (0.70%w/w), and