~ 5 ~ The Pharma Innovation Journal 2020; 9(5): 05-08 ISSN (E): 2277- 7695 ISSN (P): 2349-8242 NAAS Rating: 5.03 TPI 2020; 9(5): 05-08 © 2020 TPI www.thepharmajournal.com Received: 04-03-2020 Accepted: 06-04-2020 Yogesh P Pancham Department of Pharmaceutical Quality Assurance, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research, JNMC Campus, Nehru Nagar, Belagavi, Karnataka, India Girish B Department of Pharmaceutical Quality Assurance, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research, JNMC Campus, Nehru Nagar, Belagavi, Karnataka, India Shailendra Suryawanshi Sanjay Department of Pharmaceutical Chemistry, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research, JNMC Campus, Nehru Nagar, Belagavi, Karnataka, India Corresponding Author: Yogesh P Pancham Department of Pharmaceutical Quality Assurance, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research, JNMC Campus, Nehru Nagar, Belagavi, Karnataka, India UV-Spectrophotometric method for quantification of ascorbic acid in bulk powder Yogesh P Pancham, Girish B and Shailendra Suryawanshi Sanjay Abstract Ascorbic acid is a potent reducing and antioxidant agent that functions in fighting bacterial infections, in detoxifying reactions. In the present research work an attempt has been made to develop and validate spectrophotometric method for determination of ascorbic acid in bulk powder form using UV-1900 model. The method was developed using methanol: water (50:50 v/v) as a solvent in which ascorbic acid shows maximum absorbance wavelength at 258nm. In order to optimize the developed method, validation of ascorbic acid were performed as per ICH guidelines parameters such as Linearity, Selectivity, Specificity, Precision, Robustness, Ruggedness, LOD & LOQ, Solution stability were performed. Ascorbic acid Showed linear response between concentration range of 3, 6, 9, 12, 15μg/mL with r 2 value 0.997 all the validation report of Precision, robustness, ruggedness and solution stability were found to be in acceptance limit. Newly developed and validated spectrophotometric method was found to be selective, specific, linear, precise, robust, rugged and stable for determination of ascorbic acid in bulk form using UV-1900. Keywords: Ascorbic acid, anti-oxidant, UV-1900, ICH-guideline, stability 1. Introduction Ascorbic acid is a six carbon compound related to glucose. It is found naturally in citrus fruits and many vegetables. (Drug bank) L-Ascorbic acid is a white to very pale yellow crystalline powder with a pleasant sharp acidic taste almost odourless. Ascorbic acid is a potent reducing and antioxidant agent that functions in fighting bacterial infections, in detoxifying reactions, and in the formation of collagen in fibrous tissue, teeth, bones, connective tissue, skin, and capillaries [1] . It is available in synthetic form and well as found naturally in many medicinal plants which includes Indian gooseberry, amla citrus fruits like limes, oranges and lemons, green leafy vegetables [2] which is responsible for potent antioxidant activity. Amla is one of the major source of Ascorbic acid. Literature survey has been done for the content estimation of ascorbic acid in amla. Analytical methods such as UPLC (Klimczak I et al., 2015) [3] HPLC (Saikh M. et al., 2013) [4] were reported for estimation of Ascorbic acid in bulk. No UV-Spectrophotometric method was reported for content estimation of ascorbic acid in amla as well as in bulk form. Hence in this research work an attempt has been made to develop and validate UV-Spectrophotometric method by using standard ascorbic acid and apply the same for content estimation of ascorbic acid in amla extract. Fig 1: Chemical Structure of Ascorbic acid