Archives of Biotechnology and Biomedicine Open Access HTTPS://WWW.HEIGHPUBS.ORG 001 ISSN 2639-6777 Abstract Trichomonas vaginalis could be seen in Pap smears where it is reported, but because main concerned is placed on malignant cells in Pap smears, not much effort is done to search for this parasite in smears. In this study, 100 cervical and vaginal specimens were examined microscopically by the conventional Papanicolaou method and liquid base cytology (LBC) for the presence of Trichomonas vaginalis (T.vaginalis). 16% were infected with T.vaginalis while 10% of diagnosis based on both perinuclear halo and T. vaginalis presence although the association between perinuclear halo and T, vaginalis is statistically insignificant it is recommended to increase the number of the cases to prove or disprove the association. Research Article Perinuclear halo indicate Trichomonas vaginalis in Pap smear Howaida Mahmoud Hagag 1,2 , Yosra Hussein Alam-Eldin 3 , Mahmoud Khalifa Mahmoud 4 , Khadiga Ahmed Ismail 1.3 *, Amany Mamdouh Abdulaziz 5 , Ahmed Mahmoud Khalifa 6 and Osama Mahmoud Khalifa 7 1 Laboratory Medicine Department, Faculty of Applied Medical Sciences, Taif University, Taif, Saudi Arabia 2 Pathology Department, Faculty of Medicine, Al-Azhar University, Cairo, Egypt 3 Medical Parasitology Department, Faculty of Medicine Ain Shams University, Cairo, Egypt 4 Consaltant of Dermatology and Venereology King Faisal specialized Hospital, Taif, Saudi Arabia 5 Pathology Department, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt 6 Forensic and Toxicology Department Faculty of Medicine, Ain-Shams University, Cairo, Egypt 7 Faculty of Medicine, Ain-Shams University, Cairo, Egypt *Address for Correspondence: Khadiga Ahmed Ismail, Laboratory Medicine Department, Faculty of Applied Medical Sciences, Taif University, Taif, Saudi Arabia, Tel: 009660507921103; Email: khadigaahmed68@yahoo.com Submitted: 06 December 2018 Approved: 10 January 2019 Published: 11 January 2019 Copyright: © 2019 Hagag HM, et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited Keywords: Papanicolaou smears; Trichomonas vaginalis; Microscopy How to cite this article: Hagag HM, Alam-Eldin YH, Mahmoud MK, Ismail KA, Abdulaziz AM, et al. Perinuclear halo indicate Trichomonas vaginalis in Pap smear. Arch Biotechnol Biomed. 2019; 3: 001- 005. https://doi.org/10.29328/journal.abb.1001013 Introduction Trichomoniasis is one of the most common sexually transmitted infections (STIs) but it has received little attention and has been trivialized. Despite being a readily diagnosed and treatable sexually transmitted disease (STD). World Health Organization estimated that there were 276.4 million cases in 2008 and nearly 90 % of these infections occurred among people living in resource-limited settings [1]. Presumably, prevalence of infection with trichomoniasis is not a reportable infection, and control of the infection has received relatively little emphasis from public health STD control programs, however, appreciation of the high rates of disease and of associations of trichomoniasis in women with adverse outcomes of pregnancy and increased risk for human immunodeϐiciency virus HIV infection suggest a need for increased control efforts [2]. In Islamic countries, the prevalence of trichomoniasis ranges from 1.2% in Libya and Jordanto 3.2% in Turkeyto 28.1% in Saudi Arabia [3]. Trichomoniasis is more common in women than men because men have asymptomatic infections. For women, the symptoms are thin frothy, green-yellow vaginal discharge, vulvovaginal irritation, vaginal soreness, and redness of the vagina, women also have a higher prevalence of invasive cervical cancer when they have trichomoniasis [4].