Research Article Silver Nanoparticles Stabilized by Poly (Vinyl Pyrrolidone) with Potential Anticancer Activity towards Prostate Cancer Ahmed A. H. Abdellatif , 1,2 Ahmed Abdelfattah , 3 Abdellatif Bouazzaoui , 4,5,6 Shaaban K. Osman , 2 Issa Saad Al-Moraya , 7,8 Abdulaziz M. Saleh Showail , 9 Mansour Alsharidah , 10 Ashraf Aboelela , 11 Osamah Al Rugaie , 12 Tarek M. Faris , 13 and Hesham M. Tawfeek 3 1 Department of Pharmaceutics, College of Pharmacy, Qassim University, Buraydah, Qassim 51452, Saudi Arabia 2 Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Al-Azhar University, Assiut 71524, Egypt 3 Department of Industrial Pharmacy, Faculty of Pharmacy, Assiut University, Assiut, Egypt 4 Department of Medical Genetics, Faculty of Medicine, Umm Al-Qura University, Makkah 21955, Saudi Arabia 5 Science and Technology Unit, Umm Al-Qura University, Makkah 21955, Saudi Arabia 6 Medical Clinic, Hematology, Oncology, University Hospital Regensburg, Franz-Josef-Strauß-Allee 11, Regensburg 93053, Germany 7 Clinical Toxicology, College of Medicine Umm Al-Qura University, Makkah 21955, Saudi Arabia 8 Forensic Medicine & Toxicology Center, Ministry of Health, Abha, Saudi Arabia 9 Department of Urology, Khamis Mushait General Hospital, Ministry of Health, Khamis Mushait, Saudi Arabia 10 Department of Physiology, College of Medicine, Qassim University, Buraydah 51452, Saudi Arabia 11 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Sphinx University, Assiut, Egypt 12 Department of Basic Medical Sciences, College of Medicine and Medical Sciences, Qassim University, Unaizah, P.O. Box 991, Al Qassim 51911, Saudi Arabia 13 Department of Pharmaceutics and Industrial Pharmacy, College of Pharmacy, Al-Azhar University, Cairo 11884, Egypt Correspondence should be addressed to Ahmed A. H. Abdellatif; a.abdellatif@qu.edu.sa and Hesham M. Tawfeek; heshamtawfeek@aun.edu.eg Received 21 March 2022; Accepted 1 September 2022; Published 30 September 2022 Academic Editor: Wilson Aruni Copyright © 2022 Ahmed A. H. Abdellatif et al. is is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Tumor necrosis factor (TNF-α) and inflammatory cytokine (IL-6) play a vital role in various cellular incidents such as the proliferation and death of cells during carcinogenesis. Hence, regulation of these biomarkers could be a promising tool for controlling tumor progression using nanoformulations. Silver nanoparticles-poly (vinyl pyrrolidone) (AgNPs-PVP) were prepared using the reduction of silver nitrate and stabilized with PVP. ey are characterized through yield percentage, UV-VIS, FT-IR, size, charge, and morphology. e obtained AgNPs were tested for anticancer activity against prostate cancer (PC 3) and human skin fibroblast (HFS) cell lines. Moreover, biomarker-based confirmations like TNF-α and IL-6 were estimated. e synthesized AgNPs-PVP were stable, spherical in shape, with particle sizes of 122.33 ± 17.61 nm, a polydispersity index of 0.49 ± 0.07, and a negative surface charge of -19.23 ± 0.61 mV. In vitro cytotoxicity testing showed the AgNPs-PVP exhibited antiproliferation properties in PC3 in a dose-dependent manner. In addition, when compared to control cells, AgNPs-PVP has lower TNF-α with a significant value ( * p < 0.05); the value reached 16.84 ± 0.71 pg/ml versus 20.81 ± 0.44 pg/ml, respectively. In addition, HSF cells showed a high level of reduction ( *** p < 0.001) in IL-6 production. is study suggested that AgNPs-PVP could be a possible therapeutic agent for human prostate cancer and anti-IL-6 in cancerous and noncancerous cells. Further studies will be performed to investigate the effect of AgNPs-PVP in different types of cancer. Hindawi Bioinorganic Chemistry and Applications Volume 2022, Article ID 6181448, 12 pages https://doi.org/10.1155/2022/6181448