www.iajpr.com Page520 Indo American Journal of Pharmaceutical Research, 2019 ISSN NO: 2231-6876 DEVELOPMENT AND VALIDATION OF RP-HPLC AND TLC METHODS FOR SIMULTANEOUS ESTIMATION OF PARACETAMOL, THIOCOLCHICOSIDE AND ACECLOFENAC IN BULK AND COMBINED PHARMACEUTICAL DOSAGE FORM Ramzya I. Elbagary¹ ,2 , Ehab F. Elkady 2 , Magda M. Ibrahim 3 and Shaymaa M. Goubran 3* 1 Pharmaceutical Chemistry Department, Future University in Egypt, Cairo 12311, Egypt. 2 Pharmaceutical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr el Aini Street, 11562 Cairo, Egypt. 3 Department of Analytical Chemistry, National Organization for Drug Control and Research (NODCAR), Cairo, Egypt. Corresponding author Dr. Magda M. Ibrahim Assistant professor, Department of Analytical Chemistry, National Organization for Drug Control and Research (NODCAR), Cairo, Egypt. Mag-samaty@hotmail.com Copy right © 2019 This is an Open Access article distributed under the terms of the Indo American journal of Pharmaceutical Research, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. ARTICLE INFO ABSTRACT Article history Received 24/08/2019 Available online 30/09/2019 Keywords RP-HPLC, TLC-Densitometry, Paracetamol, Thiocolchicoside, Aceclofenac. Two accurate, precise, and sensitive methods were developed for the simultaneous determination of ternary mixtures containing paracetamol (PAR), thiocolchicoside (THIO) and aceclofenac (ACE) in bulk powder and combined dosage form without prior separation. The first method is RP-HPLC which depended on isocratic elution using an agilent C18 column and mobile phase consisting of ammonium acetate buffer (pH 7) – methanol – acetonitrile--triethylamine (50:30:20:0.1, v/v) pumped at flow rate of 1 ml/min with UV detection at 258 nm. The second method is based on TLC separation of the three drugs followed by densitometric measurements of their spots at 248, 380 and 275 nm for PAR, THIO and ACE, respectively. The separation was carried out on silica gel 60 F254 using ethyl acetate-toluene-methanol –glacial acetic acid (2:2:1:20.25 v/v/v/v) as a developing system. The suggested methods were tested using laboratory-prepared mixtures and were successfully applied for the analysis of pharmaceutical preparations. The methods retained their accuracy and precision when the standard addition technique was applied. HPLC method was applied over the concentration range (1-120 μg/ml) for Paracetamol, (0.8-60 μg/ml) for Thiocolchicoside and (10-80 μg/ml) for Aceclofenac while densitometric method was linear over the concentration range (200-10000ng/spot) Paracetamol, (10-1000ng/spot) for Thiocolchicoside and (100-10000ng/spot) for Aceclofenac, respectively. The developed RP-HPLC and TLC densitometric methods are rapid, sensitive and accurate and can be applied in quality control laboratories for routine determination of Paracetamol, Thiocolchicoside and Aceclofenac simultaneously in their ternary mixture without prior separation. Please cite this article in press as Dr. Magda M. Ibrahim et al. Development and Validation of RP-HPLC and TLC Methods f Simultaneous Estimation of Paracetamol, Thiocolchicoside and Aceclofenac in Bulk and Combined Pharmaceutical Dosage For Indo American Journal of Pharmaceutical Research.2019:9(09).