Accepted Article Does micropollutant removal by solar photo-Fenton reduce ecotoxicity in municipal wastewater? A comprehensive study at pilot scale open reactors Municipal wastewater toxicity removal by solar photo-Fenton at neutral pH Gracia Rivas Ibáñez a,b , Michal Bittner c , Zuzana Toušová c , Marina Celia Campos- Mañas a,b , Ana Agüera b , José Luis Casas López a,b , José Antonio Sánchez Pérez a,b *, Klára Hilscherová c * a Department of Chemical Engineering, University of Almería, Spain b CIESOL, Joint Centre of the University of Almería-CIEMAT, Almería, Spain c Research Center for Toxic Compounds in the Environment (RECETOX), Masaryk University, Kamenice 753/5 625 00 Brno, Czech Republic *Corresponding author: jsanchez@ual.es Tel.: +34 950015314. Fax: + 34 950015484. hilscherova@recetox.muni.cz Tel: +420-549493807 Fax +420-549492840 Abstract BACKGROUND: The reduction of toxicity in secondary effluents by solar photo- Fenton has not been widely studied. In this work, a strategy combining monitoring of micropollutants and application of a selected battery of bioassays has been applied to evaluate the effectiveness of solar photo-Fenton process for secondary effluent treatment at neutral pH in a raceway pond reactor (RPR) following two operating strategies: the sequential iron dosage and the use of the Fe(III)-EDDS complex at pilot plant scale. The applied battery of bioassays comprises in vivo (algal growth inhibition test) and in vitro, endocrine disruptors tests (androgenic/glucocorticoid activity and estrogenicity) and cytotoxicity tests. This article is protected by copyright. All rights reserved. This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi: 10.1002/jctb.5212