S94 Abstracts / Contact Lens & Anterior Eye 41S (2018) S81–S96 Methods: This is a non-delivering prospective study. Ocular sagittal height of 14 participants was evaluated with an eye pro- filer (Eaglett Eye, The Netherlands). Four different lenses (-200 m, aligned, +200 m and +400 m vs ocular sag @ 14.5 mm diameter); monocurve, Definitive material) were fitted in a random order on both eyes. Lenses were manufactured by the same lab (Microlens) and their parameters were checked with an OCT (Optimec, UK) before and after the experimentation. The lens position and move- ment were analyzed through a video technique (Aston University, UK), grading movement on push-up (mm) and the amount of drop (mm) in vertical gaze. Masked observers ranked each set of 4 lenses in order, from the least most optimal one. Comfort was evaluated with a Likert scale at insertion and after 30 min of wear. Results: There was no statistical difference for comfort at insertion (p = 0.327) or after 30 min (Friedman, p = 0.199). Push-up movement was 0.8 mm (-200 m), 0.81 (alligned and +200 m), and 0.84 (+400 m) and showed no statistical difference (ANOVA p = 0.784) However, the lens drop, when subject was asked to look upward, was near statistical difference (p = 0.057) between -200 (0.73 mm), +400 (1.2 mm) and 0 or +200 m (0.82 and 0.83 mm). Masked observers ranked the lens fitted with +200 m optimally centred for 14/28 eyes, and with optimal movement (20/28 eyes) Conclusion: Based on objective analysis, lenses fitted between 0 and 200 m of sagittal depth vs ocular sag may be considered optimally fitted. Subjective analysis tends to find lenses fitted with sag +200 m the best option. Soft lenses can be effectively fitted with ocular sag as a reference instead of central curvature of the cornea. https://doi.org/10.1016/j.clae.2018.04.044 Effects of three different daily disposable contact lenses on tear film Giancarlo Montani, Fabio Carta, Maria Rosaria Mollo Purpose: To evaluate changes induced by three different daily disposable CLs on tear osmolarity (TFO), tear meniscus height (TMH), pre lens tear film stability (PLTFS) and objective quality of vision. Methods: Forty-six subjects (new or CL wearers after a wash out period of three days) were enrolled for this open label ran- domized cross-over study. Their tear film characteristics were: NIBUT > 18 s, TMH > 18 mm and TFO < 316 mOsmol/L. Subjects were asked to wear for the first week of the study a contact lens in nesofil- con A always on the same eye and another one in delefilcon A on the fellow one. After three days of wash out it started the second week of the study with lenses in nesofilcon A again and lenses in stenfilcon A. Exams were performed at day 0 and for each CL com- bination at day 1 after 20 min and at day 7 after a minimum of 8 h of lens wear. At each examination were evaluated TMH with a slit lamp-adapted Fourier-Domain OCT, TFO with Tearlab, NIBUT and PLTFS with Easytearsview+ and objective quality of vision by means of Objective Scatter Index (OSI) for a period of 20 s between blinks measured with HD Analyzer. Results: Nesofilcon A lenses demonstrated a greater TMH after 20 min and 8h of wear (respectively 206.73 ± 48.2 m and 196.13 ± 42.4 m) than delefilcon A (171.73 ± 42.4 m and 162.93 ± 42.36 m; p < 0.05) and stenfilcon A (153.46 ± 49.7 m and 148.34 ± 53.64 m; p < 0.05). Nesofilcon A lenses demon- strated also a longer PLTFS (12.53 ± 6.27 s and 12.08 ± 5.53 s) than delefilcon A (8.33 ± 3.63 s and 9.06 ± 3.51 s; p < 0.05) and stenfilcon A (8.40 ± 3.37 s and 7.80 ± 5.26 s; p < 0.05). TFO were not significant different after 20 min and 8 h of wear with all lenses tested. Nesofil- con A lenses in respect to the other lenses tested presented also a more stable OSI over time. Conclusion: These results could be attributable to the character- istics of nesofilcon A material that allows a better water retention and wettability in comparison to the other materials tested. https://doi.org/10.1016/j.clae.2018.04.045 Eyeshadow – Polymer interaction in soft contact lenses Sara Picarazzi, Federica Corlazzoli, Alessandra Rossi, Miriam Ascagni, Stefano Farris, Alessandro Borghesi, Silvia Tavazzi Purpose: The use of cosmetics associated with soft contact lenses (CLs) made of hydrogels (Hs) and silicone-hydrogels (SHs) may cause side effects. For this purpose, the contamination of soft CLs by a purple eyeshadow was evaluated both in vitro and in vivo. Methods: A Jasco V-650 spectrophotometer was used to mea- sure the absorption of the dye component of a highly concentrated powder eyeshadow into CLs after exposure in a vial to 1 mg/mL cosmetic solution dissolved in 0.9% NaCl and after cleaning with maintenance systems. The dye absorption was compared with the mass of absorbed cosmetic including also the other cosmetic ingre- dients. In addition, analyses were performed on worn daily CLs (Delefilcon A) and worn monthly CLs (Filcon V), both worn with eyeshadow, the monthly CLs being treated with multipurpose solu- tion overnight. 10 CLs were observed by a fluorescence microscope (Olympus BX51) and 3 CLs by a confocal fluorescence microscope (Leica TCSNT). Results: From in vitro analyses, silicone-hydrogel (SH) are found to be more contaminated by the investigated eyeshadow than hydrogel (H) CLs. The diffusion of the dye component in SHs can reach 100% of the available dye in the vial, its mean value being 84% (SD 20%). Except hilafilcon A, this value is lower (p < 0.001) in Hs (19% ± 5%). As far as other ingredients are concerned, on average the absorbed mass of eyeshadow is found (667 ± 151) and (253 ± 157) g in SHs and Hs (p = 0.002), 5 mg being the available mass in the vial. Also the CLs worn by eyeshadow wearers show contam- ination, both on the surface, where dye aggregates are observed near the edge, and in depth as observed by confocal fluorescence microscopy. Surface dye deposits were detected both in daily CLs and in monthly CL (worn 7 days, multipurpose solution over night). https://doi.org/10.1016/j.clae.2018.04.046 First and third generation silicone hydrogel contact lens physical properties impact on patient preferences Jose Vega, Gary Orsborn, Steve Diamanti Purpose: Since the launch of silicone hydrogel lenses in 1999, there has been a trend toward naturally wettable, lower modu- lus, and higher water content materials in the marketplace. Studies to date have not seen a direct correlation between lens modulus and comfort. This study aimed to understand if there are comfort- related patient preference differences that correspond with lenses of different moduli and water contents after 4 weeks of daily wear. Methods: This was a prospective, double-masked, random- ized, bilateral, crossover, dispensing study comparing fanfilcon A, FA (a 3rd generation contact lens material with a modulus of 0.6 MPa, water content of 56%, and a naturally wettable surface) with lotrafilcon B, LB (a 1st generation contact lens material with a comparatively stiffer bulk modulus (1.0 MPa), lower water content (33%) and requires a surface treatment step to achieve wettability). Both lenses were worn by 47 adapted soft contact lens wearing sub- jects (37 female, 10 male) for 4-weeks each in a daily wear modality. Subjects used Opti-Free ® PureMoist ® MPS.