_____________________________________________________________________________________________________ # Senior Lecturer; ≡ Associate Professor; *Corresponding author: E-mail: arvinar.sdc@saveetha.com; Journal of Pharmaceutical Research International 33(63B): 342-350, 2021; Article no.JPRI.77924 ISSN: 2456-9119 (Past name: British Journal of Pharmaceutical Research, Past ISSN: 2231-2919, NLM ID: 101631759) Preparation of Mouthwash Using Blue Tea and Its Antioxidant Activity Ritya Mary Jibu a , Arvina Rajasekar b*# and S. Rajeshkumar c≡ a Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai-77, India. b Department of Periodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai-77, India. c Department of Pharmacology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai-77, India. Authors’ contributions This work was carried out in collaboration among all authors. All authors read and approved the final manuscript. Article Information DOI: 10.9734/JPRI/2021/v33i63B35645 Open Peer Review History: This journal follows the Advanced Open Peer Review policy. Identity of the Reviewers, Editor(s) and additional Reviewers, peer review comments, different versions of the manuscript, comments of the editors, etc are available here: https://www.sdiarticle5.com/review-history/77924 Received 24 October 2021 Accepted 27 December 2021 Published 29 December 2021 ABSTRACT Introduction: Clitoria ternatea also known as the "blue pea," is a perennial twinning herbaceous plant in the Fabaceae family. The medicinal properties of this plant have been scientifically validated, especially at an international level, and it has been recorded to have a variety of biological activities, including antioxidant, antidiabetic, and hepatoprotective properties. Aim: The aim of this study was to evaluate the antioxidant property of blue tea extract based mouthwash. Materials and Methods: The study was performed as an in vitro study under a laboratory setting. Synthesis of the mouthwash was performed using dried leaves of the butter pea plant subsequently the mouthwash was tested for its antioxidant activity and was assessed via the DPPH assay. The obtained values were compared with that of a known standard. The obtained data was sorted in Microsoft Excel and statistically analyzed using Statistical Package for Social Sciences (SPSS Software, Version 23.0). Unpaired t test was done for the comparison of blue tea extract with the standard and one way ANOVA followed by Tukey’s post hoc test was done to compare various concentrations with standard. Original Research Article