Downloaded from www.microbiologyresearch.org by IP: 54.70.40.11 On: Sat, 03 Aug 2019 11:20:49 Mucilaginibacter pineti sp. nov., isolated from Pinus pinaster wood from a mixed grove of pines trees Gabriel Paiva, 1 Pedro Abreu, 1 Diogo Neves Proenc ¸a, 1 Susana Santos, 1 Maria Fernanda Nobre 2 and Paula V. Morais 1,3 Correspondence Paula V. Morais pvmorais@ci.uc.pt 1 IMAR-CMA, University of Coimbra, 3004-517 Coimbra, Portugal 2 CNC-Center for Neuroscience and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal 3 Department of Life Sciences, FCTUC, University of Coimbra, 3004-517 Coimbra, Portugal Bacterial strain M47C3B T was isolated from the endophytic microbial community of a Pinus pinaster tree branch from a mixed grove of pines. Phylogenetic analysis of 16S rRNA gene sequences showed that this organism represented one distinct branch within the family Sphingobacteriaceae, most closely related to the genus Mucilaginibacter. Strain M47C3B T formed a distinct lineage, closely related to Mucilaginibacter dorajii KACC 14556 T , with which it shared 97.2 % 16S rRNA gene sequence similarity. The other members of the genus Mucilaginibacter included in the same clade were Mucilaginibacter lappiensis ATCC BAA-1855 T sharing 97.0 % similarity and Mucilaginibacter composti TR6-03 T that had a lower similarity (95.7 %). The novel strain was Gram-staining-negative, formed rod-shaped cells, grew optimally at 26 6C and at pH 7, and was able to grow with up to 0.3 % (w/v) NaCl. The respiratory quinone was menaquinone 7 (MK-7) and the major fatty acids of the strain were summed feature 3 (C 16 : 1 v7c/iso-C 15 : 0 2-OH), iso-C 15 : 0 and iso-C 17 : 0 3-OH, representing 73.5 % of the total fatty acids. The major components of the polar lipid profile of strain M47C3B T consisted of phosphatidylethanolamine, three unidentified aminophospholipids, one unidentified aminolipid and three unidentified polar lipids. The G+C content of the DNA was 40.6 mol%. On the basis of the phylogenetic analysis and physiological and biochemical characteristics we propose the name Mucilaginibacter pineti sp. nov. for the novel species represented by strain M47C3B T (5CIP 110632 T 5LMG 28160 T ). The genus Mucilaginibacter of the family Sphingobac- teriaceae, phylum Bacteroidetes, was first described by Pankratov et al. (2007) to group Gram-staining-negative, non-spore-forming and non-motile rods producing large amounts of extracellular polymeric substances. The genus was proposed to include two novel species, Mucilaginibacter paludis and Mucilaginibacter gracilis. At the time of writing, the genus Mucilaginibacter comprises 22 species with validly published names: M. paludis and M. gracilis (Pankratov et al., 2007), M. kameinonensis (Urai et al., 2008), M. daejeo- nensis (An et al., 2009), M. ximonensis (Luo et al., 2009), M. oryzae (Jeon et al., 2009), M. rigui (Baik et al., 2010), M. gossypii and M. gossypiicola (Madhaiyan et al., 2010), M. frigoritolerans, M. lappiensis and M. mallensis (Ma ¨nnisto ¨ et al., 2010), M. boryungensis (Kang et al., 2011), M. composti (Cui et al., 2011), M. dorajii (Kim et al., 2010), M. myungsuensis (Joung & Joh, 2011), M. angelicae (Kim et al., 2012a), M. litoreus (Yoon et al., 2012), M. lutimaris (Kim et al., 2012b), M. polysacchareus (Han et al., 2012), M. soli (Jiang et al., 2012) and M. jinjuensis (Khan et al., 2013). Members of the genus Mucilaginibacter have been isolated from very different environments including peat bogs, soils, dried rice straw, wetland freshwater (An et al., 2009; Baik et al., 2010; Madhaiyan et al., 2010; Pankratov et al., 2007; Urai et al., 2008) and also cold environments (Ma ¨nnisto ¨ et al., 2010). Pinus pinaster trees from a mixed grove of pines in Malhada, Oliveira do Hospital, Portugal, were sampled and the bark and sapwood of each cross-section were removed under sterile conditions for the study of the diversity of the endophytic community. Strain M47C3B T , forming mucoid, light-pink colonies, was isolated from dilutions of wood chips plated on R2A agar (Difco) incubated at 25 u C, for three days. The strain was maintained in the same medium supplemented with 15 % (v/v) glycerol at 280 u C after subculture and purification. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain M47C3B T is KF483876. Two supplementary figures are available with the online version of this paper. International Journal of Systematic and Evolutionary Microbiology (2014), 64, 2223–2228 DOI 10.1099/ijs.0.057737-0 057737 G 2014 IUMS Printed in Great Britain 2223