The Journal of Engineering and Exact Sciences – jCEC, Vol. 06 N. 04 (2020) journal homepage: https://periodicos.ufv.br/ojs/jcec doi: 10.18540/jcecvl6iss4pp0498-0504 OPEN ACCESS – ISSN: 2527-1075 EVALUATION OF MAGNETIC CARRIERS EMPLOYED FOR IMMOBILIZATION OF LIPASE FROM CANDIDA RUGOSA AVALIAÇÃO DE SUPORTES MAGNÉTICOS UTILIZADOS PARA IMOBILIZAÇÃO DA LIPASE DE CANDIDA RUGOSA O. DOMINGUES 1,* , L. K. SANTOS 2 , R. D. HERCULANO 1 , D. L. FLUMIGNAN 2,3 and A. V. PAULA 1 1 Department of Engineering Bioprocesses and Biotechnology, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil. 2 Department of Organic Chemistry, Institute of Chemistry, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil. 3 Campus Cuiabá, Mato Grosso Federal Institute of Education, Science and Technology (IFMT), Cuiabá, Mato Grosso, Brazil. * Corresponding author. Department of Engineering Bioprocesses and Biotechnology, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil, Phone: +55 16 993338879 e-mail address: otavio_domingues2013@hotmail.com (O. Domingues). A R T I C L E I N F O A B S T R A C T Article history: Received 2020-07-24 Accepted 2020-10-20 Available online 2020-10-20 Currently, the use of magnetic nanoparticles has aroused interest in industrial processes, and the combination of their properties with the immobilization of lipases has been developed in order to produce carriers of easy separation of the reaction medium. In this context, the objective of the present study was to immobilize Candida rugosa lipase in magnetic nanoparticles, such as magnetite and maghemite, by physical adsorption and covalent bonding. The biocatalysts were evaluated by infrared spectroscopy (FTIR) and hydrolytic activity analysis. Thus, from the analyses performed, the best biocatalyst obtained was the immobilized by covalent bond in maghemite, presenting a hydrolytic activity of 174.67 U/g. p a l a v r a s - c h a v e Espectroscopia de infravermelho Imobilização de enzimas Ligação Covalente Maghemita Nanopartículas magnéticas k e y w o r d s Infrared spectroscopy Enzyme immobilization Covalent bonding Maghemite Magnetic nanoparticles R E S U M O Atualmente, o uso de nanopartículas magnéticas tem despertado interesse em processos industriais, e a combinação de suas propriedades com a imobilização de lipases tem sido desenvolvida a fim de produzir suportes de fácil separação do meio reacional. Nesse contexto, o objetivo do presente estudo foi imobilizar a lipase de Candida rugosa em nanopartículas magnéticas, como magnetita e maghemita, por adsorção física e ligação covalente. Os biocatalisadores foram avaliados por espectroscopia de infravermelho (FTIR) e análise de atividade hidrolítica. Dessa forma, a partir das análises realizadas, o melhor biocatalisador obtido foi o imobilizado por ligação covalente em maghemita, apresentando atividade hidrolítica de 174,67 U/g.