1 Circulating Tregs accumulate in omental tumors and acquire adipose-resident features Mingyong Liu 1 , Dmytro Starenki 2 , Christopher D. Scharer 3 , Aaron Silva-Sanchez 1 , Patrick A. Molina 4 , Jennifer S. Pollock 4 , Sara J. Cooper 5 , Rebecca C. Arend 6 , Alexander F. Rosenberg 7,8 , Troy D. Randall* 1 and Selene Meza-Perez* 1 *These authors contributed equally to this work. 1 Department of Medicine, Division of Clinical Immunology and Rheumatology, University of Alabama at Birmingham, Birmingham, AL USA 2 iRepertoire, Inc, Huntsville, AL USA 3 Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA USA 4 Section of Cardio-Renal Physiology and Medicine, Division of Nephrology, Department of Medicine, University of Alabama at Birmingham, Birmingham, AL USA 5 Hudson Alpha Institute for Biotechnology, Huntsville, AL USA 6 Department of Obstetrics and Gynecology, Division of Gynecologic Oncology, University of Alabama at Birmingham, Birmingham, AL USA 7 Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL USA 8 Informatics Institute, University of Alabama at Birmingham, Birmingham, AL USA Running title: Omental tumors acquire Tregs from circulation Key words: Regulatory T cells; omentum; tissue-resident Treg, TCR repertoire, anti-tumor immunity, Financial support: This work was supported by R01 CA216234 to T.D.R. and Department of Defense Early Career Investigator Award (W81XWH-18-1-0231) to R.C.A. Address correspondence to: Troy D. Randall Division of Clinical Immunology and Rheumatology 1720 2 nd Avenue South, SHEL 507, Birmingham, AL 35294-2182 FAX: 975-3322 Phone: 975-3323 Email randallt@uab.edu Conflict of interest: D.S is a senior scientist in iRepertoire, Inc. R.C.A has been on Advisory Boards for Leap Therapeutics, AstraZeneca, GSK, Merck, VBL Therapeutics, and Caris Life Sciences. All other authors do not have a conflict of interest to report. Word count: 4578 Number of Figures and Tables: 7 Downloaded from http://aacrjournals.org/cancerimmunolres/article-pdf/doi/10.1158/2326-6066.CIR-21-0880/3054831/cir-21-0880.pdf by guest on 10 March 2022