Actinokineospora soli sp. nov., a thermotolerant actinomycete isolated from soil, and emended description of the genus Actinokineospora Xia Tang, 1 3 Yu Zhou, 1,2 3 Jing Zhang, 1,3 Hong Ming, 4 Guo-Xing Nie, 5 Ling-Ling Yang, 1 Shu-Kun Tang 1 and Wen-Jun Li 1,6 Correspondence Wen-Jun Li wjli@ynu.edu.cn Shu-Kun Tang tangshukun@hotmail.com 1 Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China 2 Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China 3 Guangdong Provincial Microbial Culture Collection and Application Key Laboratory, Guangdong Institute of Microbiology, Guangzhou 510070, PR China 4 Department of Life Sciences and Technology, Xinxiang Medical University, Xinxiang 453003, PR China 5 College of Life Sciences, Henan Normal University, Xinxiang 453007, PR China 6 Key Laboratory of Biogeography and Bioresource in Arid Land, Chinese Academy of Science, Xingjiang Institue of Ecology and Geogrpahy, Chinese Academy of Sciences, U ¨ ru ´´mqi 830011, PR China A strain of thermotolerant actinomycete, designated YIM 75948 T , was isolated from a soil sample in Yunnan province, China. The strain grew at 25–55 6C (optimum 37 6C). The substrate mycelium and aerial mycelium produced on Czapek’s agar were both pale yellow to white. The diagnostic diamino acid of the cell-wall peptidoglycan was meso-diaminopimelic acid, and the whole-cell sugars were mannose, ribose, glucose, galactose and arabinose. The major fatty acids were iso-C 15 : 0 , iso-C 16 : 0 and C 16 : 0 and the predominant respiratory quinone was MK-9(H 4 ) . The polar lipids consisted of phosphatidylethanolamine, phosphatidylethanolamine with hydroxy fatty acids, diphosphatidylglycerol, phosphatidylinositol and two unidentified phospholipids. The genomic DNA G+C content was 73.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YIM 75948 T belonged in the genus Actinokineospora and that its closest relative among recognized species was Actinokineospora fastidiosa DSM 43855 T (97.6 % sequence similarity). The mean level of DNA–DNA relatedness between the novel strain and A. fastidiosa DSM 43855 T was, however, only 47.8 %. Based on the phenotypic, chemotaxonomic and phylogenetic data and the results of the DNA–DNA hybridizations, strain YIM 75948 T represents a novel species of the genus Actinokineospora for which the name Actinokineospora soli sp. nov. is proposed. The type strain is YIM 75948 T (5JCM 17695 T 5DSM 45613 T ). The description of the genus Actinokineospora is emended to reflect the fact that the genomic DNA G+C contents of A. fastidiosa DSM 43855 T and the type strain of Actinokineospora soli sp. nov. recorded in the present study fell above the range given in previous descriptions of this genus. In 1988, the genus Actinokineospora was proposed by Hasegawa, with Actinokineospora riparia NRRL B-16432 T as the type strain of the type species (Hasegawa, 1988a, b). Subsequently, Labeda et al. (2010) proposed not only an emended description of the genus, to accommodate those Actinokineospora species that are unable to produce motile spores, but also the transfer of Amycolatopsis fastidiosa to 3These authors contributed equally to this work. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain YIM 75948 T is JN005785. A supplementary table and three supplementary figures are available with the online version of this paper. International Journal of Systematic and Evolutionary Microbiology (2012), 62, 1845–1849 DOI 10.1099/ijs.0.035832-0 035832 G 2012 IUMS Printed in Great Britain 1845