Health, 2016, 8, 1645-1653 http://www.scirp.org/journal/health ISSN Online: 1949-5005 ISSN Print: 1949-4998 DOI: 10.4236/health.2016.815160 December 6, 2016 A Simple Method for Detection of Multiple Chemical-Specific IgGs in Serum Based on Dot Blotting Mayumi Tsuji 1* , Hsu-Sheng Yu 1,2 , Yasuhiro Ishihara 3 , Toyohi Isse 1 , Nami Ikeda-Ishihara 4 , Takuto Tuchiya 1 , Toshihiro Kawamoto 1 1 Department of Environment Health, University of Occupational and Environmental Health, Kitakyusyu, Japan 2 Department of Food Science, College of Agriculture, National Pingtung University of Science and Technology, Taiwan 3 Laboratory of Molecular Brain Science, Graduate School of Integrated Arts and Sciences, Hiroshima University, Higashi-Hiroshima, Japan 4 Division of Gene Research, Natural Science Center for Basic Research and Development, Hiroshima University, Higashi-Hiroshima, Japan Abstract Plastic resins are known to cause occupational allergies. Therefore, serum-specific antibodies against plastic resins have been widely investigated as diagnostic markers for occupational allergies. In this study, we aimed to establish a convenient method for detection of multiple chemical-specific IgG antibodies in human serum based on dot blot analysis. Toluene diisocyanate (TDI), phthalic anhydride (PA), and formal- dehyde (FA), which are frequently used to synthesize various resins, reacted well with lysine residues of human serum albumin (HSA) under alkaline conditions. Na- tive polyacrylamide gel electrophoresis (PAGE) showed that the structures of chemi- cal adducts of HSA were different from those of native HSA. Therefore, we performed dot blot assays using these adducts as artificial antigens. Serum samples from workers at plants utilizing plastic resins strongly reacted with TDI, PA, and FA adducts in HSA, while reduced signals were detecting using the serum from unexposed workers. These results suggested that dot blot assays using chemical-HSA adducts as antigens could be beneficial for simultaneously measuring multiple chemical-specific IgGs. Keywords IgG, Dot Blot Assay, Plastic Resin 1. Introduction The production and consumption of plastics have increased worldwide. In 2010, plastic How to cite this paper: Tsuji, M., Yu, H.-S., Ishihara, Y., Isse, T., Ikeda-Ishihara, N., Tuchiya, T. and Kawamoto, T. (2016) A Simple Method for Detection of Multiple Chemical-Specific IgGs in Serum Based on Dot Blotting. Health, 8, 1645-1653. http://dx.doi.org/10.4236/health.2016.815160 Received: July 29, 2016 Accepted: December 3, 2016 Published: December 6, 2016 Copyright © 2016 by authors and Scientific Research Publishing Inc. This work is licensed under the Creative Commons Attribution International License (CC BY 4.0). http://creativecommons.org/licenses/by/4.0/ Open Access