Pre-clinical validation of an RNA-based precision oncology platform for patient-therapy alignment in a diverse set of human malignancies resistant to standard treatments Prabhjot S. Mundi *,1,2 , Filemon S. Dela Cruz* ,3 , Adina Grunn 1 , Daniel Diolaiti 3 , Audrey Mauguen 4 , Armaan Siddiquee 3 , Daoqi You 3 , Ron Realubit 1 , Charles Karan 1,2 , Michael V. Ortiz 3 , Melissa Accordino 2,5 , Zvika Aloni 2 , Frances Brogan 2 , Jeffrey Bruce 2,6 , Cristina I. Caescu 1 , Richard Carvajal 2,7 , Katherine Crew 2,5 , Guarionex Decastro 2,8 , Mark Heaney 2,5 , Brian Henick 2,5 , Dawn Hershman 2,5,9 , June Hou 10 , Fabio Iwamoto 2,11 , Joseph Jurcic 2,5 , Pokala R. Kiran 2,7 , Michael Kluger 2,7 , Teri Kreisl 12 , Nicole Lamanna 2,5 , Andrew Lassman 2,11 , Emerson Lim 2,5 , Gulam Manji 2,5 , Guy McKhann 2,6 , James McKiernan 2,8 , Alfred Neugut 2,5,9 , Kenneth Olive 2,5 , Todd Rosenblat 2,5 , Gary Schwartz 2,5 , Catherine Shu 2,5 , Michael Sisti 2,6,13,14 , Ana Tergas 10 , Reena Vattakalam 2 , Mary Welch 2,11 , Sven Wenske 2,8 , Jason Wright 2,10 , Hanina Hibshoosh 2,15 , Kevin Kalinsky 16 , Mahalaxmi Aburi 1 , Peter A. Sims 1,17 , Mariano Alvarez #,18 , Andrew L. Kung #,3 and Andrea Califano #,1,2,5,17,19,20 . * These authors contributed equivalently 1 Department of Systems Biology, Columbia University Irving Medical Center, 1130 Saint Nicholas Ave, New York, NY USA 10032. 2 Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center, 1130 Saint Nicholas Ave, New York, NY USA 10032. 3 Department of Pediatrics, Memorial Sloan Kettering Cancer Center, 1275 York Ave, New York, NY USA 10065. 4 Department of Epidemiology and Biostatistics, Memorial Sloan Kettering Cancer Center, 1275 York Ave, New York, NY USA 10065. 5 Department of Medicine, Columbia University Irving Medical Center, 630 W 168th Street, New York, NY USA 10032. 6 Department of Neurological Surgery, Columbia University Irving Medical Center, 710 W 168th Street, New York, NY USA 10032. 7 Department of Surgery, Columbia University Irving Medical Center, 622 W 168th Street, New York, NY USA 10032. 8 Department of Urology, Columbia University Irving Medical Center, 160 Fort Washington Ave, New York, NY USA 10032. 9 Department of Epidemiology, Columbia University Mailman School of Public Health, 722 West 168th St. NY, NY 10032 10 Department of Obstetrics & Gynecology, Columbia University Irving Medical Center, 622 W 168th Street, New York, NY USA 10032. 11 Department of Neurology, Columbia University Irving Medical Center, 710 W 168th Street, New York, NY USA 10032. 12 Novartis Five Cambridge, MA 02142, United States 13 Department of Otolaryngology Head and Neck Surgery, Columbia University Irving Medical Center, 710 W 168th Street, New York, NY USA 10032. 14 Department of Radiation Oncology, Columbia University Irving Medical Center, 161 Fort Washington Avenue, New York, NY 10032, United States 15 Department of Pathology and Cell Biology, Columbia University Irving Medical Center, 630 W 168th Street, New York, NY USA 10032. 16 Winship Cancer Institute of Emory University and Department of Hematology and Medical Oncology, Emory University School of Medicine, 1365-C Clifton Road NE, Atlanta, GA 30322, United States 17 Department of Biochemistry & Molecular Biophysics, Columbia University Irving Medical Center, 701 W 168th Street, New York, NY USA 10032. 18 DarwinHealth Inc. New York 19 Department of Biomedical Informatics, Columbia University Irving Medical Center, 622 W 168th Street, New York, NY USA 10032. 20 J.P. Sulzberger Columbia Genome Center, Columbia University Irving Medical Center, 622 W 168th Street, New York, NY USA 10032. Correspondence to: alvarez@darwinhealth.com , kunga@mskcc.org, and ac2248@columbia.edu (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint this version posted October 3, 2021. ; https://doi.org/10.1101/2021.10.03.462951 doi: bioRxiv preprint