Materials Sciences and Applications, 2017, 8, 873-888
http://www.scirp.org/journal/msa
ISSN Online: 2153-1188
ISSN Print: 2153-117X
DOI: 10.4236/msa.2017.812064 Nov. 10, 2017 873 Materials Sciences and Applications
Biochemical, Biophysical and Mechanical
Characterization of Decellularized Dermal
Implants
Frederick H. Silver
1
, Dale DeVore
2
, Ruchit Shah
3
1
Department of Pathology and Laboratory Medicine, Robert Wood Johnson Medical School, Rutgers, the State University of New
Jersey, Piscataway, NJ, USA
2
DV Consulting Services, Chelmsford, MA, USA
3
Graduate Program in Biomedical Engineering, Rutgers, the State University of New Jersey, Piscataway, NJ, USA
Abstract
Allografts have been used in a variety of applications to augment as well as re-
place tissues throughout the body. A number of steps are involved in selec-
tion, harvesting, processing and testing of dermal allografts. Grafts can be ob-
tained that are: free of antibodies to viruses and low in viral titers. Cellular
material can be eliminated from the tissue and the product becomes almost
exclusively a collagen fiber network. The purpose of this paper is to examine
the changes in collagen structure and properties that occur during processing
of decellularized dermis. The results indicate that collagen fiber swelling oc-
curs during processing although the product preserves the native collagen
banding pattern at the fibrillar structural level. Fiber swelling and decreased
collagen deformability of processed dermis, may lead to stress concentration
at the implant-tissue interface and up-regulation of mechanotransduction.
This may lead to premature mechanical failure due to creation of a chronic
inflammatory condition at the implant-tissue interface. It is suggested that all
dermal allografts be oriented such that Langer’s lines of the implant match
those of the host tissue, and that wound closure by suturing be done under
conditions that preserve the normal tension in skin in order to minimize im-
plant-interfacial failure.
Keywords
Collagen, Dermis, Repair, Wound Healing, Optical Coherence Tomography,
Vibrational Analysis, Resonant Frequency, Modulus, Langer’s Lines,
Mechanotransduction
How to cite this paper: Silver, F.H., De-
Vore, D. and Shah, R. (2017) Biochemical,
Biophysical and Mechanical Characteriza-
tion of Decellularized Dermal Implants.
Materials Sciences and Applications, 8,
873-888.
https://doi.org/10.4236/msa.2017.812064
Received: August 8, 2017
Accepted: November 7, 2017
Published: November 10, 2017
Copyright © 2017 by authors and
Scientific Research Publishing Inc.
This work is licensed under the Creative
Commons Attribution International
License (CC BY 4.0).
http://creativecommons.org/licenses/by/4.0/
Open Access