~ 2825 ~ International Journal of Chemical Studies 2020; 8(1): 2825-2829 P-ISSN: 2349–8528 E-ISSN: 2321–4902 www.chemijournal.com IJCS 2020; 8(1): 2825-2829 © 2020 IJCS Received: 07-11-2019 Accepted: 09-12-2019 Devajani Deka Department of Veterinary Public Health & Epidemiology, College of Veterinary Sciences & AH, Central Agricultural University, Selesih, Aizawl, Mizoram, India P Roychoudhury Department of Veterinary Mocrobiology, College of Veterinary Sciences & AH, Central Agricultural University, Selesih, Aizawl, Mizoram, India E Motina Department of Veterinary Public Health & Epidemiology, College of Veterinary Sciences & AH, Central Agricultural University, Selesih, Aizawl, Mizoram, India H Bayan Department of Veterinary Surgery & Radiology, College of Veterinary Sciences & AH, Central Agricultural University, Selesih, Aizawl, Mizoram, India Corresponding Author: H Bayan Department of Veterinary Surgery & Radiology, College of Veterinary Sciences & AH, Central Agricultural University, Selesih, Aizawl, Mizoram, India Detection of antimicrobial drug resistance in Listeria monocytogenes of cattle origin Devajani Deka, P Roychoudhury, E Motina and H Bayan DOI: https://doi.org/10.22271/chemi.2020.v8.i1aq.8696 Abstract The objective of this work was to investigate the antimicrobial drug susceptibility of Listeria monocytogenes strains detected from 200 samples of cattle origin by conventional bacteriology and species specific PCR. A total of 13 (6.50%) L. monocytogenes strains were detected from faeces (2.00%), offals/ internal organs (6.00%), raw meat (4.00%) and farm water (14.00%). The L. monocytogenes strains were found to be susceptible to to most of the antimicrobial drugs tested. Highest resistance to Penicillin (23.08%) followed by Ciprofloxacin (15.39%) and Nalidixic acid and Ampicillin (7.07%) was detected in L. monocytogenes strains. However, no multidrug resistant L. monocytogenes was detected in the present study. The detection of antimicrobial resistant L. monocytogenes in food animals and their environment indicates that these strains may be transferred to consumers via the food chain leading to compromise in the effective treatment of human listeriosis. Keywords: Listeria monocytogenes, antimicrobial drug, cattle, Aizawl, Mizoram Introduction Food borne infections and intoxications remain as one of the major global concern in the field of food security and food safety. Animal origin foods are considered to be the most common source of zoonotic diseases. Listeria monocytogenes is a major cause of zoonotic listeriosis and food borne infection. Infections with this bacterium are currently associated with the highest fatality rate of approximately 17% among foodborne pathogens (EFSA, 2012; Wu et al., 2015) [7] . The global outbreaks in steady rates of increase after 1980s led the World Health Organization (WHO) to conclude that L. monocytogenes is an environmental contaminant mainly transmitted to humans through food (Granier et al., 2011) [10] . The organism is hardy in nature which can grow in wide range of temperature, pH and osmotic pressure allowing its survival for longer duration in the adverse environmental conditions (Adzitey and Huda, 2010) [1] . It is also important to note that Listeria is a widely distributed bacterium in nature and commonly found in soil, sewage, dust, water and causes listeriosis in humans and animals (Norton et al., 2001) [17] . Outbreak and sporadic cases of human listeriosis have been associated with contamination of various food items milk, meat and meat products (Sur et al., 2012) [24] and pork (Goulet et al., 2012) [9] . However, outbreaks of food borne infections are not well documented in developing countries including India. A poor hygienic practice in local food production and processing chain favours the entry and transmission of different food borne pathogens. In India, L. monocytogenes should be absent in 25 gram of frozen and canned meat and fish products as per food safety and standard regulation, 2011. The organism has been isolated from reproductive disorders and mastitis of cattle, buffalo, sheep and goat (Kaur et al., 2007; Rawool et al., 2007; Shakuntala et al., 2006) [13, 19, 20] from different parts of India. Little is known about L. monocytogenes antimicrobial resistance, especially for non- human-origin isolates from India more particularly from the North Eastern region. The food production sector of animal origin in North Eastern region of India is still un-organized and there is ample scope of contamination of food with bacterial pathogens. The present work was undertaken to study the antimicrobial drug susceptibility of L. monocytogenes strains isolated from different samples of cattle origin and their environment from Aizawl district of Mizoram.