Vol.:(0123456789) 1 3
Journal of Food Measurement and Characterization
https://doi.org/10.1007/s11694-019-00224-2
ORIGINAL PAPER
Respiration rate and shelf‑life study of Crotalaria longirostrata
(chipilín)
Dolores Rovira
1
· Claudia Alfaro
1
· Violeta Martínez
1
· Isela Menjívar
1
Received: 5 February 2019 / Accepted: 24 July 2019
© Springer Science+Business Media, LLC, part of Springer Nature 2019
Abstract
The respiration rate and the shelf-life were studied on Crotalaria longirostrata, an edible leaf cultivated on the Central
American region, locally known as chipilín. Besides being a native plant in El Salvador, chipilín is a commercialized food
product. In order to obtain experimental data of respiration rates, a closed chamber approach was employed at temperatures
of 6, 20 and 30 °C. The experimental data obtained was ft to a two-parameter non-exponential equation to obtain the res-
piration rates in terms of CO
2
and O
2
changes over time. The gas concentration results were also ft to an enzyme kinetics
respiration model of the Michaelis–Menten type. Furthermore, the respiration rates from the enzyme kinetics model were
used to determine the temperature efect on the respiration. This study also provides results of the shelf-life of the unpacked
and packed chipilín with diferent materials, low density polyethylene (LDPE) and polypropylene (PP), by using a sensory
panel and ftting data to the Weibull’s probabilistic distribution. Overall results show that the enzyme kinetics model, based
on uncompetitive inhibition of CO
2
, applies very well to chipilín. The shelf life study results indicated that packaging in
LDPE and PP bags increased chipilín shelf life by 3.7 and 2.5 times respectively, compared to storage without packaging.
Keywords Respiration rate · Enzyme kinetics · Weibull hazard · Shelf life · Crotalaria longirostrata · Chipilin
Introduction
In recent years, the integration of international trades and
the growth in emigration has increased the demand of nos-
talgic foods that come from diferent parts of the world. For
instance, Tanner [1] found out that during 2013 there was an
18.4% growth in the demand for specialty products of Latin
and Mediterranean cuisine and a 13.1% increase in shelf-
stable fruits sales in the United States, and suggested that
this upward trend may continue and spread to other regions
over the next few years.
In addition, almost 20% of Salvadorans are living abroad
[2] and their potential interest on consuming their native
food, represents a favorable scenario to export food products
from El Salvador, particularly some native fruits and vegeta-
bles. However, the satisfactory export of these products is
highly dependent on storage during the postharvest handling.
Therefore, the improvement of the existing storage technolo-
gies becomes of interest to increase shelf-life.
A Salvadoran edible leaf that is very common and highly
commercialized in the Central American region is Crota-
laria longirostrata, locally known as chipilín. According to
Martinez [3] and Elias [4], this is a 1.5 meters edible veg-
etable native to El Salvador that grows in clay loams and
sandy soils and can be found in an altitude ranging between
10 and 1960 meters above sea level and a temperature range
of 16–32 °C. Martinez [3] highlights the large contents of
calcium, iron, thiamine, ribofavin and ascorbic acid and its
sizable production in El Salvador, which was reported as
more than three thousand tones between 2007 and 2008 [5].
Fresh products are perishable commodities and generally
have short shelf-life. Their deterioration is associated with
the physiological and biochemical activities and starts at the
harvesting time [6]. Living cells of harvested plants respire
continuously, utilizing oxygen (O
2
) and releasing carbon
dioxide (CO
2
). The respiration rate depends, among other
parameters, on the storage temperature and the concentration
of O
2
and CO
2
. Generally, there is an inverse relationship
between respiration rates and the postharvest life of fresh
vegetables [7].
* Dolores Rovira
mrovira@uca.edu.sv
1
Departamento de Ingeniería de Procesos y Ciencias
Ambientales, Universidad Centroamericana José Simeón
Cañas, San Salvador, El Salvador