Edumatsains,5 (2) Januari 2021, 153-164 EduMatSains Jurnal Pendidikan, Matematika dan Sains http://ejournal.uki.ac.id/index.php/edumatsains *Correspondence Address E-mail: siti.lani789@gmail.com SKRINING FITOKIMIA DARI SENYAWA METABOLIT SEKUNDER BUAH JAMBU BIJI MERAH (Psidium guajava L.) Siti Nurlani Harahap 1* , Nurbaity Situmorang 2 1,2 Prodi Ilmu Gizi Universitas Nahdlatul Ulama Sumatera Utara Diterima: 22 Oktober 2020 Direvisi: 27 Desember 2020 Diterbitkan : 10 Januari 2021 ABSTRACT Guava (Psidium guajava L.) is a tropical plant come from southern Mexico and Central America. Red guava fruit has many benefits, one of them is to control fruit fly pests on plants. In red guava fruit is presumed to contain secondary metabolite compounds can be used as natural attractants for fruit fly pests. This study aims to determine the content of secondary metabolite compounds found in red guava (Psidium guajava L.). This research used the qualitative experimental methods. The research started with processing red guava fruit samples into the powder of simpilisa. The powder of simplicia was then macerated with methanol as a solvent at a ratio of 1: 3 for + 5 days. The maceration result was extracted again by rotary evaporator to obtain the concentrated extract. The concentrated extract was screened for phytochemicals to determine the metabolite compounds contained in the sample. The results showed that the secondary metabolites in red guava fruit (Psidium guajava L.) were positive for flavonoids, alkaloids, tannins / phenolics, terpenoids / steroids. whereas the saponin test showed negative results. We can concluded that guava fruit extract (Psidium guajava L.) contains flavanoids, alkaloids, terpenoids / steroids and tannins / phenolic. Keywords: screening, phytochemicals, metabolites secondary PENDAHULUAN Tanaman jambu biji dikenal dengan nama latin Psidium guajava L., termasuk suku myrtaceae. Tanaman jambu biji berbentuk perdu, memiliki banyak cabang. Tinggi tanaman dapat mencapai lima meter. Batang berkulit cokelat dan licin. Pada kulit yang mengelupas akan terlihat kulit yang terkesan basah. Daun tanaman berbentuk oval, agak kaku, panjang sekitar 10 cm dan lebar sekitar 6 cm. Bunga kecil, berwarna putih, muncul dari ketiak daun. Buah jambu biji berukuran sebesar telur itik, berdaging buah tebal, berkulit tipis, selagi muda berwarna hijau dan setelah tua menjadi kekuning – kuningan. Buah muda berasa sepet dan setelah masak berasa manis. Biji berjumlah banyak, berbentuk kecil, bulat, keras dan terdapat di dalam daging buah (Cahyono, 2010). Secara botanis tanaman jambu biji diklasifikasikan sebagai berikut: Kingdom : Plantae Divisi : Spermatophyta Subdivisi : Angiospermae Kelas : Dicotyledonae Ordo : Myrtales Famili : Myrtaceae