43    European Journal of Biotechnology and Bioscience Online ISSN: 2321-9122 www.biosciencejournals.com Volume 4; Issue 2; February 2016; Page No. 43-47 EPR signals in plant systems and their informational content for environmental studies 1 Aygun Nasibova, 1, 2 Rovshan Khalilov, 1 Uzeyir Qasumov, 3 Boris Trubitsin, 3 Alexander Tikhonov 1 Institute of Radiation Problems, ANAS 2 Baku State University Azerbaijan. 3 Moscow State University named after M.V. Lomonosov, Moscow. Abstract The influence of increased background radiation on the formation of magnetic nanoparticles of iron oxide (magnetite – Fe 3 O 4 and maghemite – γ- Fe 2 O 3 ) in the dominant plant species of Absheron Peninsular has been investigated by EPR method. It was shown that the intensity of a broad EPR signal, characterizing the magnetic nanoparticles of iron oxide, registered in dried leaves of studied plants, collected in various times of the year noticeably increases with increasing radiation background. Also in two investigating species of plants, it has been shown that the intensity of the broad EPR signal of seeds has been much lower than in their leaves. Such behavior has been observed with control plants, and also plants growing on polluted radionuclide soils (55 and 130 mR/hour). The elemental composition, radionuclide composition and specific activity of radionuclides in the studied plants have been determined. Keywords: Radioactive contamination, exposure dose, magnetic nanoparticles, EPR signals. 1. Introduction It is known that radioactive contamination occurring in environment under the influence of wastes of chemical products and industry, also other anthropogenic factors break ecological equilibrium from serious danger for lively organisms. Recently, connecting with the contaminated environment by radioecological factors, the investigation of these factors influence on lively organisms, induces to a great interest for scientists. As known, the contamination of soils and water by oil production and gas industry is the ecological problem [1, 2] . In some areas of Absheron Peninsular the increased natural background radiation is still remains as ecological problem. In accordance with that, the radioecological studies have been conducting in these areas for a long years [3, 4] . The territory of Iodine Factory (at present it doesn’t exist) locating in Ramana districs of Absheron Peninsular is the place of our investigation. We conducted comparative experiments with control and plants exposing to chronic gamma radiation, in order to study radioactive contamination influence on the formation of magnetic nanoparticles in some plant species, growing in this territory. In the work [5] the magnetic nanoparticles accumulate in plants having grown in soils of this territory (territory of Iodine Factory) by electron paramagnetic resonance method. In the present work, the influence of increased background radiation on the formation of magnetic nanoparticles in dominant plant species having grown in the areas of Iodine Factory locating in the Absheron Peninsular in various times of the year. 2. Materials and methods 2.1. Collection of plant samples We collected leaves and seeds of plants (Elaeagnus L., Juncus L., Zygophyllum L.) on environmentally clean (control) and radioactive contaminated areas. During the investigation of radioactive contaminated territory, the exposure dose (PED) was measured by radiometer-dosimeter MKS-AT 1125, Atomtex (Belarus). Investigations were conducted on dominant species of the Apsheron peninsula having grown on radionuclide contaminated soils and on control plots. The irradiation dose rate of control plants did not exceed 10 mR/hr. Investigations were conducted on freshly collected and dried at 60 °C plants. The investigations have been conducted in some dominant plant species Elaeagnus L. (elaeagnus), Juncus L. (rush), Zygophyllum L. (bean caper) in Absheron Peninsular, having grown on radioactive contaminated and control areas (fig.1). A