International Journal of Modern Science and Technology, 2018;3(7):161-165. ISSN: 2456-0235. www.ijmst.co Research Article Received: 19.07.2018; Received after Revision: 28.07.2018; Accepted: 28.07.2018; Published: 31.07.2018 ©2018 The Authors. Published by G. J. Publications under the CC BY license. 161 An Invitro study of Anti-Inflammatory, Antioxidant and Antimicrobial Potential of Artemisia pallens N.V. Kanimozhi¹, N. Balaji² , * 1 Centre for Biotechnology, Anna University, Chennai-600 025. India. 2,* Department of Biotechnology, Madha Engineering College, Chennai-600 069. India. *Corresponding author‟s e-mail: nbalachem@yahoo.com Abstract Artemisia pallens, commonly known as Davana, is an aromatic herb found abundantly in humid habitats in the plains all over India. The plant is accredited with anthelmintic, antipyretic and tonic properties and also considered as a good fodder. Phytochemical screening of this herb showed the presence of various phytoconstituents. Extracts were analyzed for their antimicrobial capacity against bacterial strains and fungal strains and their zone of inhibition was calculated. It was done by well diffusion method. Also anti-inflammatory activity was verified i.e., protein denaturation technique is used to evaluate the anti-inflammatory activity of the extract. Finally antioxidant capacity of the ethanol extract of Artemisia pallens was investigated by phosphomolybdenum method. The extract showed strong antioxidant activities indicating that Artemisia pallens extract functions an efficient antioxidant to scavenge free radicals and reduces free radical induced cellular damage. Keywords: Artemisia pallens; Antimicrobial; Anti-inflammatory; Protein denaturation; Phosphomolybdenum method. Introduction Artemisia pallens is a small and aromatic herbaceous plant which is native to the southern part of India, especially to the states of Karnataka, Tamilnadu, Andhra Pradesh and in Maharashtra. Genus Artemisia popularly known as “Sage Brush” or “Worm wood” is bitter aromatics [1]. Artemisia pallens is utilized in traditional ayurvedic medicinal formulations. Essential oil of davana is useful as antiseptic and disinfectant. Oral administration of methanol extract of this extract led to significant blood glucose lowering effect in glucose fed hyperglycemic and alloxan induced diabetic rats studied [2]. Artemisia pallens commonly known as “Davana” has been traditionally used in Indian folk medicine for the treatment of diabetes mellitus, wound healing and immunomodulating, antihelmintic, antipyretic, antibacterial, antifungal, tonic properties and stimulant. It is also considered as good fodder [3]. In current study the anti-inflammatory activity was investigated by Protein denaturation technique. There were no reports about the antioxidant and antimicrobial activity of stem extract of Artemisia pallens. Hence, in the present study, the ethanolic extract of Artemisia pallens was screened for antioxidant and antimicrobial activities. Materials and methods Chemicals used Ethanol, Ethyl acetate, Petroleum ether, Ferric chloride, Sodium hydroxide, Sulphuric acid, Fehling's solution A, B, Hydrochoric acid, Meyer's reagent, Olive oil, ᾰ naphthol, Benedicts reagent, Ninhydrin, Tetracycline, Ammonium molybdate, Sodium phosphate, Sulphuric acid, Egg albumin, and Phosphate buffer. Instruments/apparatus required Magnetic stirrer, Weighing balance, Heating mantle, Aluminium foil, Petri plates, Micro pipettes, and Colorimeter. Test organisms used Bacillus subtilis, Escherichia Coli, Klebsiella pneumoniae, Staphylococcus aureus, Trichoderma sp., and Candida albicans.