Sigara içen ve içmeyen bireylerde orta derecede egzersizin oksidatif stres üzerine akut etkileri 1 Gazi University, School of Medicine, Department of Clinical Biochemistry 2 Gazi University, School of Medicine Department of Anesthesiology & Reanimation, 3 Hacettepe University Faculty of Sports Science ABSTRACT Aim: : One of the particular sources of oxidative stress is smoking and thiobarbituric acid reactive substances (TBARS) and malondialdehyde (MDA) have been used as biomarkers of lipid peroxidation of oxidative damage. Therefore, we aimed to investigate the acute effect of moderate exercise on oxidative stress by determining serum MDA levels by TBARS in moderate smoker subjects versus non-smokers. Material and Methods: Fifty healthy subjects performing moderate intensity exercise were assigned to 2 groups as moderate smokers (11-20 cigarettes/day) (Group Smoker, n=25) and non-smokers (Group Non-Smoker, n=25). Venous blood samples were collected from all participants half an hour before exercise (pre-exercise) and immediately after exercise (post- exercise) to determine MDA levels as an indicator of lipid peroxidation in the serum by TBARS/UV (ultra violet). Results: Although no significant differences were observed in pre-exercise MDA levels between the groups, post-exercise MDA levels in smokers were significantly higher than that of non-smokers (p<0.05). Conclusion: The MDA determination in serum by TBARS/UV appears to be positively correlated with smoking status in particularly female subjects. Therefore, it can be a promising helpful tool in demonstrating the oxidative stress due to moderate exercise particularly in smokers to reorganize a healthier life style. Key words: Malondialdehyde (MDA), Smoking, Exercise, Oxidative Stress Acute efect of moderate exercise on oxidative stres in smoker versus non-smokers Turkish Journal of Clinics and Laboratory Corresponding Author * : Berrin GUNAYDIN, MD, PhD, Gazi University, School of Medicine Department of Anesthesiology & Reanimation E-Mail: gunaydin@gazi.edu.tr Recevied 14.02.2018 accepted 22.02.2018 Doi: 10.18663/tjcl.394776 Banu CAYCI 1 , Berrin GUNAYDIN* 2 , Seher YUKSEL 1 , Sibel SOYLEMEZ 1 , Cagrı ALTUNDARAK 3 To cite this article: Cayci B, Gunaydin B, Yuksel S, Soylemez S, Altundarak C. Acute effect of moderate exercise on oxidative stres in smoker versus non- smokers. Turk J Clin Lab 2018; 9(1): 55-58. 55 Original Article