~ 3282 ~ Journal of Pharmacognosy and Phytochemistry 2018; 7(3): 3282-3283 E-ISSN: 2278-4136 P-ISSN: 2349-8234 JPP 2018; 7(3): 3282-3283 Received: 01-03-2018 Accepted: 05-04-2018 Mudasir A Mir Division of Plant Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology (SKUAST-K), Shalimar, Srinagar, Jammu and Kashmir, India Zahoor Dar Department of Biotechnology, University of Kashmir, Srinagar, Jammu and Kashmir, India P Mani Department of Biotechnology, Annai College of Arts & Science, Kumbakonam, Tamil Nadu, India Waseem Bhat Oxford College of Science, HSR Layout Bangalore, Karnataka, India MV Rao Department of Plant Science, School of Life Sciences. Bharathidasan University. Tiruchirappalli, Tamil Nadu India Correspondence Mudasir A Mir Division of Plant Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology (SKUAST-K), Shalimar, Srinagar, Jammu and Kashmir, India In-vitro anti-proliferative activity of Sambucus wightiana Mudasir A Mir, Zahoor Dar, P Mani, Waseem Bhat and MV Rao Abstract Sambucus wightiana is an important traditional medicinal plant that is used to relieve various health related issues. The demand for natural anti-cancer agents has gained a lot of momentum from last few years. Therefore, current research work was carried out to evaluate In-vitro anti-proliferative activity against breast cancer cell line (MCF-7) using MTT based assay. Nevertheless, results have showed that at tested concentrations, the cells did not show any significant anti-proliferative activity (IC50> 1000 μg/ml). Keywords: anticancer, MCF-7, Sambucus wightiana, MTT assay Introduction Sambucus wightiana Wall. ex Wight & Arn. (Adoxaceae) is a perennial woody shrub with various ethno-medicinal properties e.g. to initiate vomiting for expelling poisonous substances, treat stomach disorders [1] , as laxative, to treat skin diseases, as diuretic, anti-inflammatory, expectorant, diaphoretic, hypertensive and also as dye for coloring yarn [2] . The ethno- medicinal plants finds their application towards agricultural, cosmeceutical, food industries and also for addressing different diseases including life-threatening diseases like AIDS, Cancer, Diabetes [3] . Medicinal and aromatic plant species possesses various bioactive molecules that have potential to prevent and treat different diseases including cancers. The anti-cancer drugs are mostly natural in origin as 60% market based drugs are from natural sources [4, 5] . It is important to screen more and more number of natural sources such as plants to discover lead small biomolecules against prominent cancers. Among all cancer types, breast cancer is considered as a major cause of death among women of developed and developing countries [6] . Therefore, current investigation was carried out to evaluate In-vitro anti-proliferative activity of leaf portion of Sambucus wightiana against deadly breast cancer cell line. Materials and Methods Biological materials and culture medium Sambucus wightiana leaf samples were collected from Ahribal region of Kashmir Himalayan region and voucher specimen was deposited at Centre for Biodiversity and Taxonomy, University of Kashmir herbarium (KASH-1732). The shade dried sample was proceeded for powder formation and phytochemical extraction was done using methanol [7] . Dried crude methanol extract was dissolved in distilled DMSO and stock solution of 1 mg/ml concentration was made. The serial dilutions were made to prepare different concentrations of the extract (62.5, 125, 250, 500 & 1000 μg/ml) to carry out cytotoxic studies. Determination of In-vitro cytotoxicity activity The cytotoxicity study (MTT assay) was carried out as described previously [8] . Results and Discussion The results of in-vitro cytotoxicity and anticancer activity of methanolic leaf extract of Sambucus wightiana are presented in Table 1 and Fig.1. The morphological changes in two selected cell lines (L6- normal rat muscle and MCF7-human breast carcinoma) were observed by microscopic examination which revealed no marked cytotoxicity in the given extract concentration range against these two cell lines. The IC 50 values against both the cell lines were found to be >1000μg/ml. The capacity of cells to resist toxic shock has remained the foundation of most cytotoxicity assays and MTT assay is based on the principle that dead cells or their products do not reduce tetrazolium. The assay depends both on the number of cells