Annales Pharmaceutiques Françaises (2020) 78, 142—149 Disponible en ligne sur ScienceDirect www.sciencedirect.com ORIGINAL ARTICLE Formulation and clinical evaluation of the standardized Litchi chinensis extract for skin hyperpigmentation and aging treatments Formulation et évaluation clinique d’un extrait de Litchi chinensis pour l’hyperpigmentation et les effets de l’age sur la peau N. Lourith a,*,b , M. Kanlayavattanakul a,b a School of Cosmetic Science, Mae-Fah-Luang University, 57100 Chiang-Rai, Thailand b Phytocosmetics and Cosmeceuticals Research Group, Mae-Fah-Luang University, 57100 Chiang-Rai, Thailand Received 15 August 2019; accepted 13 January 2020 Available online 23 January 2020 HIGHLIGHTS • The stable 0.05 and 0.1% litchi serums were formulated and clinical evaluated. • Skin lightening efficacy of the litchi serum was significantly improved. • Skin elasticity and wrinkle reduction of the litchi serum was significantly achieved. • The efficacies on forearm and facial skin were conformed. • Litchi extract is clinically confirmed for skin lightening and anti-aging. KEYWORDS Litchi chinensis; Skin lightening; Skin aging; Skin barrier function; Cosmetics Abstract Introduction. — The standardized litchi extract had been revealed on phytochemical actives, in vitro and cellular activities against aging and darkening of skin. However, a formulation containing the extract has never been developed as per clinical evaluated. Materials and methods. — The litchi serum was developed, safety and efficacy were clinically evaluated in human volunteers. The stable and none irritated 0.05 and 0.1% litchi serums were randomized-single blind placebo control clinical applied on the inner forearm of 29 volunteers for a consecutive 112 days and monitored by Mexameter ® MX18, Cutometer ® MPA 580 and Visioscan ® VC 98. ∗ Corresponding author. E-mail address: nattayal@mfu.ac.th (N. Lourith). https://doi.org/10.1016/j.pharma.2020.01.004 0003-4509/© 2020 Acad´ emie Nationale de Pharmacie. Published by Elsevier Masson SAS. All rights reserved.