~ 86 ~ The Pharma Innovation Journal 2019; 8(5): 86-91 ISSN (E): 2277- 7695 ISSN (P): 2349-8242 NAAS Rating: 5.03 TPI 2019; 8(5): 86-91 © 2019 TPI www.thepharmajournal.com Received: 23-03-2019 Accepted: 24-04-2019 Snehal S Manekar Student, Government College of pharmacy, Amravati, Maharashtra, India Manoj S Charde Assistant Professor, Department of Chemistry, Government College of pharmacy, Amravati, Maharashtra, India Correspondence Snehal S Manekar Student, Government College of pharmacy, Amravati, Maharashtra, India Evaluation of anti-asthmatic activity of Lawsonia inermis Linn. Aerial parts Snehal S Manekar and Manoj S Charde Abstract The aim of present study is to evaluate the antiasthmatic potential of aerial parts of plant of Lawsonia inermis Linn. Using several experimental models. The dried and powdered aerial parts of Lawsonia inermis was extracted with continuous soxhlet extraction with Petroleum ether (40-60 0 C), Chloroform, Ethyl acetate, Acetone, Methanol and Hydroalcoholic solvents. Preliminary phytochemical screenings of all extracts were done. Preliminary animal studies by In-vitro isolated goat trachea chain preparation of all extracts were done to find out potent extract. In this study, the methanolic extract of aerial parts of Lawsonia inermis was found to be potent comparative to other extract. The results of carrageenan induced rat paw oedema model indicated the dose dependant anti-inflammatory activity. As compared to standard drug (Indomethacin), methanolic extract showed similar activity which were found to be statistically significant (P<0.0001). The extent of DPPH radical scavenging was determined by calculated the IC50 value of methanolic extract Lawsonia inermis (101.8) compared with the Ascorbic acid (114.7) taken as standard. In the present study the histamine induced dose dependent contraction of goat tracheal chain was significantly inhibited (p< 0.001) by methanolic extract of aerial parts of Lawsonia inermis (200 μg/ml). Thus the present study revealed that the methanolic extract of Lawsonia inermis (MEBP) has significant antihistaminic (H1 receptor antagonist) activity. In view the fact that tribal have well experience the antiasthmatic effects of the roots of Lawsonia inermis Linn. The results of our study, for the first time, show that the methanolic extract of aerial parts of Lawsonia inermis Linn. Possesses antioxidant, anti-inflammatory, Bronchodilator properties and therefore can be used for the antiasthmatic treatment. Keywords: Anti-asthmatic, Antihistaminic, Anti-inflammatory, Lawsonia inermis. Aerial parts Introduction Asthma is a dynamic, chronic disorder of the lungs involving airway obstruction, caused by inflammation and hyperresponsiveness. Asthma is a very common chronic condition. Its prevalence varies worldwide but more than 5% of any investigated population suffers from asthma. In some regions this percentage is much higher. Asthma affects all ages: it is the most common chronic disease of childhood, adolescence and adulthood and affects patients in their most productive years [1] . The use of Lawsonia inermis L. (henna) for medicinal and cosmetic purposes is inextricably linked to ancient and modern cultures of Asia. It has been traditionally reported in use of headache, hemicranias, lumbago, bronchitis, boils, ophthalmia, syphilitis, sores, amenorrhea, scabies, diseases of the spleen, dysuria, bleeding disorder, skin diseases, diuretic, antibacterial, antifungal, anti-amoebiasis, astringent, anti-hemorrhagic, hypotensive and sedative effect. The plant is reported to contain Lawsone, Esculetin, Fraxetin, Isoplumbagin, Scopoletin, Betulin, Betulinic acid, Hennadiol, Lupeol, Lacoumarin, Laxanthone, Flavone glycosides, two pentacytic triterpenes [2] . Materials and methods Plant materials The aerial parts of plant Lawsonia inermis were collected from the outfield of Amravati city, Maharashtra, India in month of November- December. The plant materials (Ref. No- Gvish/BOT/Report/10/2015) were identified and authenticated by Dr. S.N. Malode, Head, P.G. Department of Botany, Govt. Vidarbha Institute of Science and Humanities, Amravati (M.S.), India. Preparation of extract of Lawsonia inermis linn. The dried and powdered aerial parts of Lawsonia inermis (500 gm) were extracted with