1 Extrachromosomal circular DNAs (eccDNAs), Amplified Oncogenes, and CRISPR–Cas9 System Fatemeh Pourrajab 1, 2, 3, 4* , Mohamad Reza Zare-Khormizi 5, 6 1 Reproductive Immunology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran 2 Nutrition and Food Security Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran 3 Hematology & Oncology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran 4 Biotechnology Research Center, International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran, 5 School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran, 6 Cardiovascular Research Center, Kerman University of Medical Sciences, Kerman, Iran, Corresponding authors: 1- Fatemeh Pourrajab, Reproductive Immunology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. E-mail: mina_poorrajab@yahoo.com (F. Pourrajab). Running title: Extrachromosomal circular DNAs (eccDNAs) and CRISPR–Cas9 System This article has not been copyedited and formatted. The final version may differ from this version. Molecular Pharmacology Fast Forward. Published on August 8, 2022 as DOI: 10.1124/molpharm.122.000553 at ASPET Journals on October 30, 2022 molpharm.aspetjournals.org Downloaded from