Decoding ribosomal RNA modification dynamics at single molecule resolution Oguzhan Begik 1,2,3,# , Morghan C Lucas 1,4,# , Jose Miguel Ramirez 1 , Ivan Milenkovic 1,4 , Sonia Cruciani 1,4 , Helaine Graziele Santos Vieira 1 , Rebeca Medina 1 , Huanle Liu 1 , Aldema Sas-Chen 5 , John S Mattick 3 , Schraga Schwartz 5 and Eva Maria Novoa 1,2,3,4,6* 1 Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Dr. Aiguader 88, Barcelona 08003, Spain 2 Garvan Institute of Medical Research, Darlinghurst, NSW, 2010, Australia 3 UNSW Sydney, Darlinghurst, NSW, 2052, Australia 4 Universitat Pompeu Fabra (UPF), Barcelona, Spain 5 Weizmann Institute of Science, Rehovot, IL 6 Lead Contact # These authors contributed equally * Correspondence to: Eva Maria Novoa (eva.novoa@crg.eu) (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint this version posted July 7, 2020. ; https://doi.org/10.1101/2020.07.06.189969 doi: bioRxiv preprint