Int.J.Curr.Microbiol.App.Sci (2020) 9(11): 176-184 176 Original Research Article https://doi.org/10.20546/ijcmas.2020.911.021 SSR Markers based Molecular Assessment of Stripe Rust Resistance Genes in F3 Population of Cross DBW17 x WH1105 of Wheat (Triticum aestivum L.) Vijeta Gupta * , Mukesh Kumar, Vikram Singh and Lakshmi Chaudhary Department of Genetics & Plant Breeding, College of Agriculture, Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana, India *Corresponding author ABSTRACT Introduction Wheat (Triticum aestivum L.) has been defined as the „King of cereals‟ because of the acreage, high productivity and the prominent position it holds in the international food grain trade. Wheat is a disomic allohexaploid (2n = 6x = 42, AABBDD) crop with seven pairs of chromosomes derived from three different genomes A, B and D and has an extremely large genome size of 17 × 10 9 base pairs with more than 80% repetitive DNA. During 2018-19, the area, production, productivity of India was 29.72 mha, 98.61 mt and 3.32 t/ha, respectively (Anonymous, 2019). Wheat production is constrained not merely due to limited natural resources and changing climate but to a great extent by emergence of new more virulent pathotypes of economically important pathogens. Multiple diseases can attack wheat crop but the fungal disease yellow rust caused by a biotroph International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 9 Number 11 (2020) Journal homepage: http://www.ijcmas.com To evaluate the presence of resistance genes parents (DBW17 and WH1105) and F3 population of cross DBW17 x WH1105 were screened for reaction to stripe rust using Modified Cobb‟s Scale and using SSR markers. Considering the inheritance pattern of resistance to yellow rust a ratio of 13:3 was observed for resistant to susceptible plants. For genotyping 99 primers were used out of which 15 primers were found to be polymorphic and were dispersed over the wheat genome (AABBDD), with allele range 2-5. A total of 32 alleles were detected in polymorphic markers. The average number of SSR alleles per locus was 2.13, with a range from 2 to 4. PIC values of various SSR loci ranged from 0.44 to 0.66. The hierarchical cluster analysis revealed that the F3 population along with their parents were mainly divided into two major clusters, I and II at a similarity coefficient of 0.54. Dendrogram confirmed that two parents were highly diversed and all of the progeny lines were interspersed between the two parents. These parents can be used for developing RIL‟s that could be used for QTL mapping to identify the resistance genes and chromosome location. Keywords F3 population, SSR markers, Resistance, Stripe rust and Wheat Accepted: 04 October 2020 Available Online: 10 November 2020 Article Info