~ 206 ~ Journal of Pharmacognosy and Phytochemistry 2016; 5(1): 206-210 E-ISSN: 2278-4136 P-ISSN: 2349-8234 JPP 2016; 5(1): 206-210 Received: 20-11-2015 Accepted: 23-12-2015 Lokadi Pierre Luhata Laboratoire de Phytochimie et contrôle qualité, ISAV- Kimwenza, 3724, Kinshasa, The Democratic Republic of Congo (DRC). Namboole Moses Munkombwe Department of Chemistry, University of Zambia, 32379, Lusaka, Zambia Hanzooma Hatwiko Department of Pharmacy, University of Zambia, 32379, Lusaka, Zambia Correspondence Lokadi Pierre Luhata Laboratoire de Phytochimie et contrôle qualité, ISAV- Kimwenza, 3724, Kinshasa, The Democratic Republic of Congo (DRC). Isolation and 1 H-NMR identification of a tiliroside from Odontonema strictum (Acanthaceae) Lokadi Pierre Luhata, Namboole Moses Munkombwe, Hanzooma Hatwiko Abstract The dried leaves (310g) of Odontonema strictum (OSM) were poured and extracted in MeOH/DCM (1/1//v/v) for 48 hours to yield 25g of dry residue which were purified using chromatographic methods (vacuum liquid chromatography column (VLC), Sephadex LH-20 column and Thin Layer Chromatography (TLC)). Fractions containing flavonoids were mixed for further purifications. Compound 1, yellow amorphous powder (8 mg) was re-dissolved in MeOH and developed in EtAOc/MeOH (2:1) to give an Rf value of 0.27 and mp 301-303°C. The compound reacted positively to HCl-Mg and Molish reactions. The 1 H-NMR (400 MHz, CDCl3) spectrum displayed the typical signals for tilirosides. The presence of glycosidic flavonoids may possibly contribute to the pharmacological properties of OSM. Keywords: Odontonema strictum, identification, flavonoids, tilirosides, 1H NMR spectroscopy. Introduction OSM (Figure 1), a plant mostly found in tropical regions, is used as a folk medicine in Burkina-Faso to treat hypertension [1] . Felix Kini and his colleagues [2] have identified flavone glycosides (C-heterosides and O-heterosides in 0.37% and 1.13% respectively) as the active secondary metabolites. In our previous work, we have isolated stigmasterol and beta- sistosterol from OSM and confirmed the antibacterial properties of these phytosterols [3-4] . Recently, the hepatoprotective and antioxidant activity of OSM against CCl4-Induced Hepatic Injury in Rats have been confirmed by M. S. Refaey and his colleagues [5] . In the continuing study on this plant, we isolated and identified a compound which belongs to the group of tilirosides (TLDs). We are still working to elucidate the complete structure of the isolated compound. TLDs are glycosidic flavonoids which exhibit several pharmacological properties such as anti-diabetic and anti-hyperlipedemic [6] , antiviral and cytotoxic activity [7] , anti– inflammatory, anti-rheumatism [8] , anti-microbial and antioxidant [9-10] . Kaempferol-3-O-β-D (6-O-transp-cinnamoyl) glucopyranoside (trans-tiliroside,) revealed significant anti- hyperglycemic effects when compared with phenethyldiguanide in alloxan mice. As a part of trans-tiliroside, kaempferol-3-O-β-D-glucopyranose and related analogues revealed weak anti- diabetes activity [11] . Structurally, Tilirosides are composed of three distinct parts: a flavonoid, a phenyl propanoid and a sugar (Figure 2). Fig 1: Odontonema strictum