International Journal of Technology 11(7)1329-1336 (2020) Received July 2020 / Revised November 2020 / Accepted December 2020 International Journal of Technology http://ijtech.eng.ui.ac.id Using Ultrasonic Assisted Extraction to Produce a Bioinsecticide from Cigarette Butt Waste and Green Solvent to Control Armyworm Infestation Faradilla Andiani Pratiwi 1 , Tania Surya Utami 1* , Rita Arbianti 1 1 Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Kampus UI Depok, Depok 16424, Indonesia Abstract. Indonesia has one of the highest rates of cigarette consumption in the world, and it has been estimated that up to 75% of cigarette butts end up in nature, where they damage the environment. Since 2019, a new species of armyworm (Spodoptera frugiperda or the fall armyworm) has been attacking maize plants in Indonesia. Therefore, it is proposed to use cigarette butts in the production of a bioinsecticide to control this armyworm. Tobacco, the main ingredient in cigarettes, contains various compounds that can be used as insecticides. These compounds can be extracted using an ultrasonic-assisted extraction method and a variety of solvents, namely, aquadest, 96% ethanol, and NADES as green solvent. The highest extract yield was 27.2±2.0% and the highest mortality rate for the fall armyworm occurred with 96% ethanol extract. The 16- Hentriacontanone compound with the highest peak area of 22.67% was obtained using a gas chromatography mass spectrometry (GC/MS) instrument. All compounds obtained from the GC/MS instrument were simulated with molecular docking to the acetylcholinesterase receptor. The highest docking score was -10.3 kcal/mol for 2,3-Dimethyl-5,6-diphenyl-1,7-dihydrodipyrrolo pyridine and 16-Hentriacontanone, which had a 100% similarity of interactions with the control ligand. Keywords: Bioinsecticides; Cigarette butt waste; Green solvent; Spodoptera frugiperda; Ultrasonic assisted extraction 1. Introduction It has been estimated that up to 75% of cigarette butts end up in nature (Cigarette Litter Organization, 2001). One way to reduce the waste from cigarette butts is to convert them into bioinsecticides. The main ingredient of cigarettes, tobacco leaves, contain various compounds that have the potential to be used as insecticides. These compounds include alkaloids, flavonoids, fatty acids, and essential oils (Khalalia, 2016; Kirkova et al, 2016). The advantages of biopesticides are that they are easily degraded, have no long-term residue, are fast acting, and have low mammalian toxicity and low phytotoxicity (Haryuni et al., 2019). The targeted pest for this bioinsecticide from cigarette butts is the fall armyworm. The loss of harvest from an attack by the Spodoptera litura armyworm can reach 80% if the species is not controlled (Marwoto and Suharsono, 2008). Larvae damage crops by biting, chewing, and then eating the lower surface of the leaves. The leaves become transparent * Corresponding author’s email: nana@che.ui.ac.id, Tel.: +62-21-7863516; Fax: +62-21-7863515 doi: 10.14716/ijtech.v11i7.4474