Vol. 11 | No. 3 |1328 - 1333 | July - September | 2018 ISSN: 0974-1496 | e-ISSN: 0976-0083 | CODEN: RJCABP http://www.rasayanjournal.com http://www.rasayanjournal.co.in Rasayan J. Chem., 11(3), 1328-1333(2018) http://dx.doi.org/10.31788/RJC.2018.1133095 KINETIC-SPECTROPHOTOMETRICDETERMINATION OF p- BROMOANILINEAT THE MICROGRAM LEVEL Kavita Rawat, Jaspal Singh*, R. D. Kaushik and Nishant Khandelwal Department of Chemistry, GurukulaKangriVishwavidyalayaHaridwar, *E-mail : sjs2874@yahoo.com ABSTRACT The determination of p-Bromoaniline(PBA) was investigated by kinetic-spectrophotometric method followed by the progress of Mn II catalyzed reduction of periodate ion in the acetone-water medium. The reaction was found to be first order with respect to the catalyst, substrate,and oxidant each. The reaction was followed by monitoring the increase in the absorbance of the reaction intermediate. The main reaction product characterized on the basis of melting point and spectroscopic studies is 4-methyl-1,2-benzoquinone. The effect of pH, dielectric constant of medium and free radical scavengers were studied to develop the best-fit conditions for developing a new and simple kinetic-spectrophotometric method for microgram determination of p-Bromoanilinein the range 0.52µ g/cm 3 to 48.37 µ g/cm 3 .The characteristics of various calibration curves, detection limits, percentage recovery,and effect of interferants, correlation coefficient and comparison of the proposed methods with other reported methods are presented in this article. Keywords: Microgram Estimation,periodate ion, Mn II catalyzed,p-Bromoaniline, 4-Bromo-1,2-Benzoquinone. © RASĀYAN. All rights reserved INTRODUCTION Aromatic amines are highly polar organic compounds that are widely used as intermediates or produced in various fields such as drugs, dyes, tobacco smoke, pesticides and pharmaceutical industries and as antioxidants in the polymer industries. 1,2 Aromatic amines are suspected to be carcinogenic and highly toxic to the human body, aquatic organisms and environment, therefore de-contamination of aromatic amines from industrial wastewater is necessary. In order to understand the behavior of aromatic amines and fate in human body and environment, highly sensitive and selective methods for the determination of aromatic amines is necessary. Many workers have reported the estimation of some aromatic amines in smaller amounts in different media and using various complex reaction systems involving complicated pre-treatment and employing various techniques like NMR, IR Spectroscopy, Spectrophotometry, Polarography, Flow injection analysis, Solvent extraction, Raman spectroscopy, and various chromatographic techniques including TLC, gas chromatography, HPLC, reversed phase HPLC, GC-MS, GC/LC, GC-ECD, ion exchange chromatography and electrochemical detection. 3-12 The only report available in the literature regarding the kinetic-spectrophotometric estimation of some anilines is based on their oxidation by periodate ion in aqueous/ mixed media. 13-14 Earlier reports have proposed that Periodate oxidation of aromatic amines has not been explored widely for kinetic spectrophotometric determination of amines and only a few attempts have been made in this direction. 15-24 Some reports are available on Evaluation of stability constant of ternary intermediate complex formation in Mn II catalyzed periodate oxidation of 2,5-xylidine by stopped flow method. 31 Some aniline was investigated as fabrication of polyaniline nanofiber modified carbon paste electrodes (GOx/MCPE) 32 . The detailed kinetic-mechanistic studies on Mn II catalyzedthe reduction of periodate by p- Bromoanilinehas also been reported. 25 This reaction has not been explored for estimation of p- Bromoaniline(PBA). Present paper deals with a new and simple kinetic-spectrophotometric method