127 LC DETERMINATION OF NATAMYCIN IN DOOGH WITH UV DETECTION Abstract A rapid and simple High-Performance Liquid Chromatographic method with UV detection (HPLC-UV) has been developed for the first time to determination of natamycin in Doogh. Doogh is a refreshing drink that is made with yogurt, water, and mint. The method is based on extraction of 10 g of Doogh with 75 mL of methanol and 25 mL of distilled water. An aliquot of 20 µl of Extract after filtration injected to HPLC. This compound was detected using a Grace Smart RP C18 column (100 x 4.6 mm, 3 µm I.D.) and the mobile phase used, was acetonitril: 30mM perchloric acid (35:65) in 5 min. The flow rate was maintained at 0.5 mL/min and the analytical signal was measured at 304 nm. HPLC-UV İLE DOOGH'DA NATAMİSİN BELİRLENMESİ Özet Doogh içinde natamisin belirlenmesi için Yüksek Performans S›v› Kromatografi yöntemi (HPLC-UV) ile basit ve h›zl› bir yöntem, ilk olarak gelifltirilmifltir. Doogh ‹ran'a özgü yo¤urt, su ve nane ile yap›lan serinletici bir içecektir. Yöntem, 10 g Doogh örne¤inin 75 mL metanol ve 25 mL saf su ile ekstraksiyonu esas›na dayanmaktad›r. 20 µL ekstrakt, filtre edildikten sonra HPLC'ye enjekte edilmifltir. Natamisin, Grace Smart RP C18 kolonunda (100 x 4.6 mm, 3 µm I.D.) belirlenmifltir ve mobil faz asetonitril:30 mM perklorik asittir (35:65). Süre 5 dakikad›r. Ak›fl h›z› 0.5 mL/ dakikad›r ve analiz 304 nm'de gerçeklefltirilmifltir. Niloofar Khoshbakht Fahim, Hamed Reza Beheshti, Javad Feizy 1 , Somayeh Sadat Fakoor Janati Testa Quality Control Laboratory, North-East food industrial technology and biotechnology park, Mashhad, Iran. GIDA (2012) 37 (3): 127-132 Research / Araflt›rma 1 Corresponding author / Yazışmalardan sorumlu yazar ;  feizy.j@gmail.com, ✆ (+90) 511 542 4393, (+90) 511 542 4396 GD12028 Received / Gelifl tarihi: 15.03.2012 Accepted / Kabul tarihi: 31.03.2012