27 Bulletin UASVM Agriculture, 66 (2)/2009 Print ISSN 1843-5246; Electronic ISSN 1843-5386 Evaluation of Storage Level for Some Heavy Metals and Others Elements into Lichens Species Growing On Larix Decidua in Bucegi Area Gabriela BUSUIOC, Claudia STIHI, Ivona DAVID, Carmen ELEKES, Nicoleta ILIESCU Universitatea VALAHIA din Targoviste, B-dul RegeleCarol I nr. 2, g1busuioc@yahoo.com Abstract: It is well known the affinity of lichens for heavy metals absorption, and used as biomonitors in environmental protection [4]. Also they are studied for their hyper accumulation level in the view to be used as natural’s sources for rare and noble metals. This work is about two lichens species which are growing on Larix decidua’s bark. It was collected and analyzed Usnea barbata and Peltigera canina. Both lichens species were growing in closer association each other on bark. The samples were analyzed by EDXRF (fluorescence –spectrometer) method with ELVA-X. In this way were registered all the elements which were in a concentration higher then 1ppm. It were determinate the concentrations of iron, copper, zinc, chromium, manganese, lead, cobalt. In some analysed cases were registered nickel, strontium and gold. Most important there were the absorption features of lichens which was different from a place to other in studied aria. In the east of this zone both lichens species had the same characteristics of storage heavy metals. Keywords: lichens, Usnea barbata, Peltigera canina, heavy metals. INTRODUCTION All over the world are increasing the researches studies about chemical content of different lichens species which are growing on bark of trees [3, 5]. American studies relieved that the large expansion of lichens is the effect of global climatic change. But another cause is the enlarge pollution of environmental components [1, 2, 4]. The lichens species studied here have the features of invaders because their large expansion on Coniferrals species. It seems to becoming parasitic organisms. In Europe in France, Romania, Russian and others country, lichens association are growing on many Coniferrals species, as Larix decidua. In our country on can see a disaster in Bucegi Mountains from this point of view. A large number of Coniferrals which are invaded by lichens associations as in case of Larix decidua. MATERIALS AND METHODS Biological material consisted in Usnea barbata and Peltigera canina collected from some Larix decidua’s bark in Busteni aria from south (Poiana Costiei), east (Poiana Izvoarelor) and north (Pichetu Rosu). Biological samples and their substrate were drying at 60°C for 1 hour. The elemental content of samples was determinate using Elva-X Spectrometer having a X-ray tube with Rh anode.The samples were excited for 300s and the characteristic X-rays were detected by a multichannel spectrometer based on a solid state Si- pin-diode X-ray detector with a 140 mm Be window and a energy resolution of 200eV at 5.9keV. In this way were registered all the elements which were in a concentration higher then 1ppm. For the evaluation of EDXRF results was used a certified reference sample (NIST SRM 1571-Orchard leaves). Every result represents the average of some determinations.