~ 41 ~ International Journal of Mosquito Research 2016; 3(5): 41-46 ISSN: 2348-5906 CODEN: IJMRK2 IJMR 2016; 3(5): 41-46 © 2016 IJMR Received: 08-07-2016 Accepted: 09-08-2016 Muhammad Mohsin (a) Health Services Academy Islamabad, Pakistan (b) Public Health Pests Laboratory, Jeddah Governate, Jeddah, Kingdom of Saudi Arabia Samina Iqbal Naz Health Services Academy Islamabad, Pakistan Imtinan Akram Khan Public Health Pests Laboratory, Jeddah Governate, Jeddah, Kingdom of Saudi Arabia Aliya Jabeen Health Services Academy Islamabad, Pakistan Hazrat Bilal Public Health Pests Laboratory, Jeddah Governate, Jeddah, Kingdom of Saudi Arabia Rizwan Ahmad Public Health Pests Laboratory, Jeddah Governate, Jeddah, Kingdom of Saudi Arabia Yonus Alshamrani Public Health Pests Laboratory, Jeddah Governate, Jeddah, Kingdom of Saudi Arabia Emad IM Khater Public Health Pests Laboratory, Jeddah Governate, Jeddah, Kingdom of Saudi Arabia Ernest Tamboi Public Health Pests Laboratory, Jeddah Governate, Jeddah, Kingdom of Saudi Arabia Correspondence Muhammad Mohsin (a) Health Services Academy Islamabad, Pakistan (b) Public Health Pests Laboratory, Jeddah Governate, Jeddah, Kingdom of Saudi Arabia Susceptibility status of Aedes aegypti and Aedes albopictus against insecticides at eastern Punjab, Pakistan Muhammad Mohsin, Samina Iqbal Naz, Imtinan Akram Khan, Aliya Jabeen, Hazrat Bilal, Rizwan Ahmad, Yonus Alshamrani, Emad IM Khater and Ernest Tambo Abstract Susceptibility status of Ae. albopictus and Ae. aegypti was evaluated in different localities of four towns namely Samanabad, Nishtar, Ravi and Iqbal town in Lahore district, Eastern Punjab, Pakistan. Susceptibility test using WHO test kits was applied on adult females (12-24) hrs. Post emergence (non- blood fed) live mosquitoes under laboratory conditions at 27-29 0 C temperature and 85-95% humidity. Ae. aegypti and Ae. Albopictus were found resistant to 4% DDT with < 36.28% mortality. Both species were also found resistant against 0.05% deltamethrin, 0.05%, lambdacyhalothrin and 0.75% permethrin at all localities with <75.00%, <87.00% and <81.05% mortalities respectively. Ae. albopictus was found resistant to 0.1% bendiocarb at Samanabad and Iqbal towns, while resistance possible to be confirmed at Nishtar and Ravi towns. Ae. aegypti found susceptible to 0.1% bendiocarb at Samanabad town, resistant at Ravi town and resistance possible to be confirmed (incipient) at Nishtar and Iqbal towns with 92.23% and 94.00% mortalities. Malathion was found most suitable insecticide with 100% mortality rate in Ae. albopictus and Ae. aegypti at Lahore district. Alternative use of malathion and bendiocarb may be the best option to reduce dengue vector density. Keywords: Aedes, albopictus, aegypti, mosquito, dengue, insecticide, resistance, control, Lahore, Pakistan 1. Introduction Dengue fever and Dengue hemorrhagic fever (DHF) are arboviral vector-borne diseases which is endemic in more than 100 countries in the WHO regions of Africa, the Americas, the Eastern Mediterranean, Western Pacific and the South-East Asia [1] . Increasingly the growing emergence and resurgence of Aedes-related dengue and neglected tropical diseases have been documented in new and old areas since 2009 according World Health Organization (WHO) report [1] . The unprecedented evolving dengue and Zika threats increased the global community concern and require concerted local and international commitment in proactive and innovative approaches and tools in tackling the global scourge. Specifically, dengue has experienced a 30-fold increase in incidence over the past 50 years that shows no sign of slowing down [2] . The Western Pacific regions and South-East Asia are the most seriously affected [1] . Recent estimates are that approximately 390-400 million people are infected each year and 96 million manifest with clinically apparent disease [2] . In Pakistan, Ae. aegypti and Ae. albopictus are suspected vectors of dengue, which are responsible for the first infection of dengue hemorrhagic fever (DHF), reported from Karachi in 1994 and so on [3, 4] . In 2006, about 5,522 dengue cases were reported by Health Directorate of Pakistan. In 2010, unprecedented flooding increased the breeding sites for mosquitoes and resulted in dengue epidemic resurgence with documented 9,000 cases and 38 deaths [5] . Moreover, 2011 witnessed the worst dengue epidemic disaster with 22,562 confirmed dengue cases and 363 deaths in Pakistan [5] . Overall from 2011‐2014, more than 48,000 laboratories confirmed dengue cases were reported across the country [6] . Integrated efforts in tackling this evolving public health burden have been relying on recommended global and national WHO pesticide evaluation scheme and other approaches and strategies. Since the introduction of organic insecticides in 1940s, chemical control has become the most important method in mosquito control [1, 7] .