Int.J.Curr.Microbiol.App.Sci (2019) 8(9): 43-53 43 Original Research Article https://doi.org/10.20546/ijcmas.2019.809.007 Postmortem Blood and Tissue Changes for Estimation of Time of Death Supriya Das 1 , S.K. Panda 1* , A.P. Acharya 1 , U.K. Mishra 2 , A.K. Kundu 3 , B.N. Mohanty 4 and I. Ali 1 1 Department of Veterinary Pathology, 2 Department of Veterinary Anatomy, 3 Department of Veterinary Physiology, 4 Department of Veterinary Parasitology, College of Veterinary Science & Animal Husbandry, Odisha University of Agriculture & Technology, Bhubaneswar, India *Corresponding author ABSTRACT Introduction After death, a sequence of changes i.e. a series of chemical as well as physical reactions naturally occur in the dead body. These changes are called as the postmortem changes. Postmortem interval (PMI) or time of death is the time that has elapsed since the animal has died. Post mortem interval estimation is very important in human as well as in animal forensic death investigations (Brooks, 2016). Despite of various research on this aspect, accuracy in estimation of time of death cannot be significantly improved and there is not a International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 8 Number 09 (2019) Journal homepage: http://www.ijcmas.com The present work was carried out in Department of Veterinary pathology, College of Veterinary Science and Animal Husbandry, OUAT, Bhubaneswar to study changes of different blood parameters in postmortem blood with respect to time passed since death. Postmortem changes of different body tissues like liver, heart, kidney etc. were also studied. Blood samples collected in EDTA vial were analysed for estimation of Hb, TEC, TLC and pH. Hb in postmortem blood had no correlation with time passed since death. But TEC, TLC and pH were strongly correlated with time passed since death. As time since death increased TLC as well as TEC decreased but pH of post-mortem blood increased. There were also some remarkable tissue changes occur in postmortem samples as time passed since death increased. These changes can help in rough approximation of time of death estimation. Keywords Hb, TEC, TLC, Time of death Accepted: 04 August 2019 Available Online: 10 September 2019 Article Info