Open Access Research Article
Ismail et al., J Pet Environ Biotechnol 2013, 4:6
DOI: 10.4172/2157-7463.1000163
Volume 4 • Issue 6 • 1000163
J Pet Environ Biotechnol
ISSN: 2157-7463 JPEB, an open access journal
Abstract
The aim of this research was to study the infuence of nanoparticles on the biodegradation of crude oil. Production
of biosurfactants was also assessed. Crude oil-utilizing bacteria were isolated from oil felds via enrichment in
chemically defned medium with crude oil as a sole carbon source. The isolates could be affliated to the genera
Bacillus, Pseudomonas, Achromobacter, and Microbacterium by 16S rRNA gene sequencing and phylogenetic
analysis. GC/FID analysis revealed 52 to 98% degradation of the oil saturate fraction within one month. Nanoparticles
of ZnO inhibited growth and crude oil biodegradation by one isolate (NBHCO4) in a concentration-dependent manner.
Growth of the cultures containing ZnO nanoparticles was relatively slower than that of the nanoparticles-free cultures.
Moreover, the oil degradation extent in the nanoparticles-containing cultures (22-50%) was less than that achieved
in cultures lacking ZnO nanoparticles (80%). Two strains, NBHCO2 and NCEOW, emulsifed and utilized water-in-oil
emulsions (chocolate mousse). Biosurfactant production in only one crude oil culture (I-19) could be confrmed by the
observed reduction in surface tension. Some isolates produced biosurfactants from water-soluble substrates such as
glucose. The NBHCO2 strain produced a lipopeptide biosurfactant which reduced the surface tension of the growth
medium from 72 to 27 mN/m. A gene of catechol dioxygenase was detected in the I-19, NBHCO4, and NCEOW
isolates indicating the potential catabolic capabilities of these strains for degradation of the aromatic oil fraction. In
conclusion, metal oxide nanoparticles can interfere with crude oil biodegradation. Biosurfactants are not necessarily
a prerequisite for crude oil biodegradation. The isolates can be applied for bioaugmentation of petroleum-polluted
soil and biosurfactants production.
Bacterial Degradation of the Saturate Fraction of Arabian Light Crude oil:
Biosurfactant Production and the Effect of ZnO Nanoparticles
Wael Ismail
1
*, Noora A Alhamad
1
, Wael S El-Sayed
2,4
, Ashraf M El Nayal
1
, Yin-Ru Chiang
3
, Riyad Y Hamzah
1
1
Biotechnology Program, College of Graduate Studies, Arabian Gulf University, Manama, Kingdom of Bahrain
2
Taibah University, Faculty of Science, Biology Department, Al-Madinah Al-Monawarah, 344, Kingdom of Saudi Arabia
3
Biodiversity Research Center, Academia Sinica, Taipei 115, Taiwan
4
Microbiology Department, Faculty of Science, Ain Shams University, Cairo, Egypt
Keywords: Bioremediation; Biosurfactant; Emulsifcation;
Nanoparticles; Bacillus
Introduction
Petroleum hydrocarbons are among the most common
environmental contaminants [1,2]. Petroleum and its hazardous
products can be released into the environment through various
production, processing, and consumption operations. Consequently,
environmental pollution with petroleum and its products has become
an issue of global concern, particularly with the anticipated boost in the
production and consumption of fossil fuels [3-5].
Pollution with petroleum hydrocarbons in the Arabian Gulf region
has become a serious environmental issue. In deed the Arabian Gulf is
one of the most heavily polluted aquatic environments worldwide. It
was estimated that ca 60% of the oil transported via the sea is shipped
in the Arabian Gulf [5,6]. Furthermore, the Arabian Gulf region has
witnessed the largest oil spill to date in terms of the amount of crude oil
discharged. During the 1991 Gulf war the release of about 11 million
barrels of crude oil has lead to the pollution of all environmental
components in the region [7].
In Kuwait, where the situation was the worst, about 190 km
of border trenches were flled with 3.5 million barrels of crude oil.
Moreover, 400 km
2
of land were polluted because of the fallout from
800 burning wells which released 50 million m
3
of crude oil [8]. Te
marine environment and ground water reservoirs were also heavily
impacted. Another devastating consequence is the formation of more
than 300 oil lakes that covered over 49 km
2
of land with more than 40
million tons of crude oil [9,10]. In addition to land and water pollution
with petroleum hydrocarbons, the 1991 Gulf war contributed massively
to air pollution [11].
Although more than two decades have elapsed since the
catastrophic Gulf war oil spill occurred, the consequences are still
apparent today [10]. Following the 1991 Gulf war, some studies have
been conducted to evaluate bioremediation as a cleanup technology in
the Arabian Gulf territory [9,12,13]. Nonetheless, the work achieved to
date is not up to the scale of the massive pollution problem. Most of the
research done has focused on the marine environment of the Arabian
Gulf. In contrast, a few studies have addressed the crude oil-degrading
microbial populations in petroleum-impacted soil.
It is well known that one of the factors which ofen impede the
biodegradation of hydrophobic substrates like hydrocarbons and crude
oil is their low bioavailability [14,15]. Tis is because these substrates
have poor aqueous solubility and therefore, are not readily accessible
for microbial attack. To circumvent this problem, microbes, which
exist predominantly in the aqueous phase, have evolved diferent
mechanisms to get access to and utilize hydrophobic compounds. Some
*Corresponding author: Wael Ismail, Biotechnology Program, College of
Graduate Studies, Arabian Gulf University, Manama, Kingdom of Bahrain, Tel:
+97336146948; Fax: +97317239664, E-mail: waelame@agu.edu.bh
Received November 05, 2013; Accepted December 20, 2013; Published
December 28, 2013
Citation: Ismail W, Alhamad NA, El-Sayed WS, El Nayal AM, Yin-Ru C, et al.
(2013) Bacterial Degradation of the Saturate Fraction of Arabian Light Crude
oil: Biosurfactant Production and the Effect of ZnO Nanoparticles. J Pet Environ
Biotechnol 4: 163. doi:10.4172/2157-7463.1000163
Copyright: © 2013 Ismail W, et al. This is an open-access article distributed under
the terms of the Creative Commons Attribution License, which permits unrestricted
use, distribution, and reproduction in any medium, provided the original author and
source are credited.
Journal of Petroleum &
Environmental Biotechnology
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ISSN: 2157-7463