124 SOLENODON 12: 124-135, 2015 Another new cryptic frog related to Eleutherodactylus varleyi Dunn (Amphibia: Anura: Eleutherodactylidae), from eastern Cuba Luis M. DíAz* and S. Blair HEDgES** *Museo Nacional de Historia Natural de Cuba, Obispo #61, Esquina Ofcios, Plaza de Armas, Ha- bana Vieja, CP 10100, Cuba. E-mail: luisfromcuba@yahoo.es **Department of Biology, 208 Mueller Laboratory, Pennsylvania State University, University Park, Pennsylvania 16802-530, USA. E-mail: sbh1@psu.edu ABStrACt. A new cryptic frog, Eleutherodactylus beguei sp. nov., is described from the pine forests of La Munición, Yateras, Guantánamo Province, Cuba. It is sympatric with E. feichtin- geri, another recently described grass frog closely related to E. varleyi, but differs in morphol- ogy, vocalization and DNA sequences of the mitochondrial Cyt-b gene. One female of the new species was found vocalizing in response to a calling male, a behavior that is still poorly documented in anurans. Same male and female were found in axillary amplexus and sur- rounded by 9 eggs (3.5–3.7 mm in diameter) 5 hours after being isolated in a small container. Key words: terrarana, Euhyas, West Indies, Guantánamo, new species, female reciprocation calls, eggs. INtrODUCtION After a recent review of the geographic variation of the Cuban Grass Frog Eleutherodactylus varleyi Dunn, Díaz et al. (2012) described E. feichtingeri, a cryptic species widely distributed in central and eastern Cuba. the two species differ primarily in tympanum size, supratympanic stripe pattern, and advertisement calls. Species recognition was also supported by genetic and cytogenetic data. One of the authors (SBH) conducted DNA sequence analyses that confrmed the existence of two species at La Munición, Humboldt National Park. One spe- cies is E. feichtingeri and the other is a new species that we describe here. Subsequently, in the summer of 2011, LMD collected additional specimens of the new taxon, made recording of vocalizations, and obtained data on ecology and behavior. MAtErIALS AND MEtHODS Morphological and bioacoustical measurements were taken as mentioned in Díaz et al. (2012). Advertisement and reciprocation calls were defned following Duellman and trueb (1986:97). A portion (~800 bp) of the cytochrome b (Cyt-b) mitochondrial gene was sequenced for four species and all molecular methods were identical to those in our previous study (Díaz et al., 2012). Calls were recorded with a Marantz PMD 661 digital sound recorder and a Sennheiser ME 66 microphone. Acoustic signals were digitized at 44 kHz and a sample size