Middle East Journal of Applied Science & Technology Vol.5, Iss.3, Pages 22-41, July-September 2022 ISSN: 2582-0974 [22] www.mejast.com Vector Bionomics, Potential Vectors, Susceptibility and Bio-efficacy of LLINs Nets on Anopheles Mosquitoes in Nanyin Village Bunmouk Township Sagaing Region in Myanmar Maung Maung Mya 1* , Myat Phone Kyaw 1 , Myat Thu Soe 2 , Pye Lin Aung 2 , Than Tun 3 , Nwet Nwet Aye 3 , Ye Kyaw Thu 2 , Swen Htat Aung 2 , Phone Myint Lwin 2 , Aung Kyaw Kyaw 2 & Kyaw Kyaw Linn 4 1 Department of Medical Research, 2 ICEMR Research Project, 3 VBDC Department of Health, 4 Township Medical Officer, Bunmouk Township, Sagaing Region. Corresponding Author Email: dr.mgmgmya@gmail.com* DOI: https://doi.org/10.46431/MEJAST.2022.5303 Copyright © 2022 Maung Maung Mya et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Article Received: 10 May 2022 Article Accepted: 19 July 2022 Article Published: 30 August 2022 1. Introduction Malaria is one of the major communicable disease-causing high mortality and morbidity among population in malaria endemic areas where tropical and sub-tropical climate zone such as Asia, Africa and South Saharan country. Malaria is high in south East Asian Regions including Myanmar. Myanmar government has committed to eliminate malaria by 2030. The national framework for malaria elimination released by the Government of Myanmar plans to achieve this goal through strategic planning in a phased manner [1]. India also plan to eliminate malaria in 2030 releases by government of India [2]. Maung Maung Mya -Research Scientist/Expert, Medical Entomology Research Division, Department of Medical Research, Ministry of Health, Myanmar, Email: dr.mgmgmya@gmail.com. Myat Phone Kyaw -Deputy Director General, Department of Medical Research, Ministry of Health, Myanmar, Email: Kyaw606@gmail.com. Myat Thu Soe -Research Officer, ICEMR Research Project, Ministry of Health, Myanmar, Email: dr.myatthusoe@gmail.com. Pye Lin Aung -Research Officer, ICEMR Research Project, Ministry of Health, Myanmar, Email: pyaelinnag@gmail.com. Than Tun -Assistant Director, Vector Born Diseases Control, Department of Health, Ministry of Health, Myanmar, Email: thantun@gmail.com. Nwet Nwet Aye -Senior Entomologist, Vector Born Diseases Control, Department of Health, Ministry of Health, Myanmar, Email: nwetnwetaye@gmail.com. Ye Kyaw Thu -Research Assistant, ICEMR Research Project, Ministry of Health, Myanmar, Email: yekyawthu@gmail.com. Swen Htat Aung -Research Assistant, ICEMR Research Project, Ministry of Health, Myanmar, Email: swenhtataung@gmail.com. Phone Myint Lwin -Research Assistant, ICEMR Research Project, Ministry of Health, Myanmar, Email: phonemyintlwin@gmail.com. Aung Kyaw Kyaw -Research Assistant, ICEMR Research Project, Ministry of Health, Myanmar, Email: aungkyawkyaw@gmail.com. Kyaw Kyaw Linn -Township Medical Officer, Bunmouk Township Hospital, Department of Health, Ministry of Health, Myanmar, Email: kyawkyawlinn@gmail.com ABSTRACT Nanyin village is a malaria endemic area consisting of Bunmouk Township Sagaing Region in Myanmar. Ohnpinkone and Bwedarkone wards were chosen for measuring vector bionomics, potential vectors, susceptibility and bio-efficacy of long lasting insecticidal nets (LLINs) (due to high prevalence of P. falciparum) in both wards from December 2019 to October 2020. A total of 3236 and 3019 Anopheles mosquitoes consist of 12 species were collected from both Ohnpinkone and Bwedarkone wards. Anopheles barbirostris was found highest number (1088 and 820) followed by An. philippinensis (267 and 605) in both areas. Higher number of Anopheles mosquitoes were collected by Cattle bait catching method followed by Indoor Light traps method. The highest was collected in October in Ohnpinkone and September in Bwedarkone. In Ohnpinkone, the highest was collected in April by outdoor and August in indoor light trap and March by outdoor and June by indoor light traps in Bwedarkone. Higher number of main vectors of An. minimus and An. dirus were collected in Ohnpinkone than Bwedarkone and highest number of An. minimus was collected by cattle bait (77) followed by indoor light method (72) in Ohnpinkone. Only 11and 3 An. dirus were collected, of this 9 and 1 number were collected by indoor light traps method from both wards. One An. minimus from Ohnpinkone was found sporozoite positive (0.47%). Main vector An. minimus was collected highest in April in Ohnpinkone and June in Bwedarkone. Susceptibility status of primary and secondary vectors were found susceptible to Pyrethroid insecticides. Bio-efficacy of all tested LLINs nets were found lower efficacy 20% and 63.33% in both wards. Main vector of An. minimus was collected year round in large number in both areas as well as sporozoite positive and An. dirus was available in both areas. Therefore, both areas are highly malaria risk areas and need to proper treatment and control measure using RIS and supply new LLINs nets or retreatment with deltamethrin to old nets according to WHO guideline to ovoid malaria transmission. Keywords: Bionomics, Susceptibility, Sporozoite, LLINs, Anopheles, Main vector, An. minimus, Bio-efficacy, Pyrethroid, Deltamethrin insecticide.