Draft Genome Sequence of a Cladosporium Species Isolated from the Mesophotic Ascidian Didemnum maculosum Anastasia Gioti, a Romanos Siaperas, b Efstratios Nikolaivits, b Géraldine Le Goff, c Jamal Ouazzani, c Georgios Kotoulas, a Evangelos Topakas b a Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for Marine Research, Heraklion, Greece b Industrial Biotechnology & Biocatalysis Group, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, Athens, Greece c Institut de Chimie des Substances Naturelles, ICSN, CNRS, Gif sur Yvette, France ABSTRACT We report the 28-Mbp draft genome sequence of the marine fungus Cladosporium sp. strain TM138. The species was isolated from the marine inverte- brate Didemnum maculosum. Its genome sequence will inform future investigations into the species’ enzymatic potential for bioremediation and its evolution in marine environments. M arine environments account for more than 95% of the total environmental biodiversity. Fungi isolated from these environments are increasingly gaining attention for their potential use in a wide spectrum of industrial applications (1) since they constitute a rich source of bioactive compounds (2, 3) and comprise an arsenal of novel enzymes that can be used in, e.g., bioremediation (1, 4). Species of Cladosporium exhibit broad physiological capacities, such as halotolerance (5), but relatively little is known about their genomes. We report here the draft genome sequence of a Cla- dosporium species isolated from a marine environment, obtained through shotgun sequencing. Cladosporium sp. strain TM138 (isolate code TM138-S3) was isolated from the yellow encrusting ascidian Didemnum maculosum, collected in March 2017 from a rock substrate in Almuñecar, Spain, at a 31-m depth (6). For isolation, a 1-cm 3 tissue piece was ground in sterile seawater and heated at 50°C for 1 h. The suspension was serially diluted, plated onto Difco marine broth agar (MBA; BD Biosciences, NJ, USA), and incubated at 28°C for 6 weeks. Pure MBA cultures were established from a single colony. For DNA extraction, the fungal biomass was filtered from 3-day-old liquid cultures (27°C and 150 rpm shaking) in Difco marine broth, and the GenElute plant genomic DNA miniprep kit (Sigma-Aldrich, MO, USA) was used. A 450-bp insert size library was prepared with the Nextera NEBNext Ultra II FS DNA library prep kit and sequenced in paired-end mode (read length, 150 bp) by Eurofins Genomics Europe Sequencing GmbH (Constance, Germany) on a NovaSeq6000 S2 instrument. Sequencing yielded 4,996,400 paired reads, totaling 1,498,920,000 bp. Adapter sequences were removed (at an alignment score above 7, allowing 2 mismatches), and bases with a quality score below 18 and below an average of 20 on a 5-bp window were trimmed using Trimmomatic v.0.39 (7). Reads smaller than 50 bases or with no pair (singletons) were discarded. Identification of contaminant reads was performed with Kraken2 v.0.8 (8), with a confidence score of 0.7 and using a custom database com- prising UniVec and RefSeq genomes from Bacteria, Archaea, viruses, plants, and fungi (accessed December 2019). Based on this analysis, read pairs that were not classified as belonging to other taxa were used for de novo assembly, invoking error correction with BayesHammer as bundled with SPAdes 3.13.0 (9, 10), using k-mers 33, 55, 77, and 99, automatic computation of the coverage threshold, and an option to minimize the number of mismatches. The SPAdes assembly was polished with one round of Pilon Citation Gioti A, Siaperas R, Nikolaivits E, Le Goff G, Ouazzani J, Kotoulas G, Topakas E. 2020. Draft genome sequence of a Cladosporium species isolated from the mesophotic ascidian Didemnum maculosum. Microbiol Resour Announc 9:e00311-20. https://doi.org/10.1128/ MRA.00311-20. Editor Antonis Rokas, Vanderbilt University Copyright © 2020 Gioti et al. This is an open- access article distributed under the terms of the Creative Commons Attribution 4.0 International license. Address correspondence to Anastasia Gioti, n.gioti@hcmr.gr. Received 25 March 2020 Accepted 14 April 2020 Published 30 April 2020 GENOME SEQUENCES crossm Volume 9 Issue 18 e00311-20 mra.asm.org 1 on July 9, 2020 by guest http://mra.asm.org/ Downloaded from