compared to urinary cytology for the diag- nosis of bladder cancer. Material and Methods: Seventy consecu- tive patients with history of non-muscle- invasive bladder cancer performed NMP22 test, cytology test and cystoscopy. All pa- tients underwent TUR of lesions. Pts with history of CIS bladder cancer were ex- cluded from our series. Results: At final histologic exam 67 NMI and 3 MI bladder cancer were found. Out of 67 NMI, 20 patients resulted Ta low grade, 27 patients resulted Ta high grade, and 20 patients resulted in T1 high grade. NMP22 ASSAY: Of the 70 tumors found, NMP22 assay was positive in 38 cases; the overall sensitivity resulted 54%. Two of 20 tumors low grade have been detected (sensitivity 10%); 36 of 50 high grade tu- mors have been detected (sensitivity 72%); 19 of 47 tumors Ta (40%); 16 of 20 tumors T1(80%): 3 of 3 tumors T2 (100%) resulted positive at NMP22. Cytology: of the 70 tumors founded cytology was posi- tive in 33 cases; the overall sensitivity re- sulted 47%.1 of 20 tumors low grade have been detected (sensitivity 5%); 32 of50 high grade tumors have been detected (sensitivity 64%); 15 of 47 tumors Ta (32%); 17 of 20 tumors T1 (85%): 1 of 3 tumors T2 (33%) resulted positive at cytol- ogy. Conclusions: Our results showed that NMP22 test has the same efficacy of urine cytology for detecting NMIBC. In our clin- ical practice its use is recommended due to lower cost and less time consuming compared to urine cytology. UP-01.033 Diagnostic Value of an Urine Based Tumor Marker for ScreeningLower Urinary Tract in Low-Risk Patients with Asymptomatic Microscopic Hematuria Sagnak L, Ersoy H, Gucuk O, Ozok U, Topaloglu H Diskapi Yildirim Beyazit Education And Research Hospital, 3rd Urology Clinic, Ankara, Turkey Introduction and Objective: To evalu- ate the use of NMP22BladderChek in com- parison by voided urine cytology for screening subjects with asymptomatic mi- croscopic hematuria (AMH); younger than 40 years who are at low risk for bladder cancer based on non-smoking history; by discussing the guidelines on this subject. Materials and Methods: From October 2005 to September 2007, 164 patients (56 male and 108 female) were included in the study for evaluation. Patients with risk factors according to AUA Best Practice Policy Recomendations on AMH were strictly excluded from the study. for up- per urinary tract imaging, ultrasonography was performed and prior to cystoscopic procedure freshly voided urine was sam- pled for urine cytology and NMP22BC assay in all patients. Biopsy was per- formed if suspicious lesions were seen or if (+) cytology was obtained. Results: The mean age was 30.8 years. As some benign urological pathologies were detected in 21 patients by ultrasonogra- phy, NMP22BC was (+) in 26 patients where the cytology was confirmed as atypia in 5. Two TaG1 tumors were de- tected cystoscopically in a 39 year-old man and a 33 year-old woman where the NMP22BC test was (+) and the cytology was (-) in both of them. NMP22BC test sensitivity, PPV and NPV were detected higher than cytology. Conclusion: We recommend that in eval- uation of low-risk patients with AMH, as initial tests, two non-invasive and cost- effective methods can be chosen: first of all, an upper tract imaging by usg as rec- ommended by guidelines, followed by NMP22BC test for lower tract investiga- tion instead of urine cytology; in a contro- versy with guidelines. UP-01.034 Expression of CD147 in Patients with Non-muscle InvasiveTransitional Cell Carcinoma of Urinary Bladder: Utility as a Marker of Presence of Tumour Seth A, Bhagirath D, Abrol N, Sharma A All India Institute of Medical Sciences, New Delhi, India Introduction and Objective: Transi- tional cell carcinoma (TCC) accounts for 90% of the tumours of the urinary blad- der, divided in to two variants: muscle invasive and non-muscle invasive. The non-muscle invasive form has a recur- rence rates exceeding 50% (Tetu B 2009), hence regular surveillance by the means of cystoscopy is essential to check for tu- mour recurrence. We need markers that can indicate presence of tumour (primary or recurrent). CD147 is a cell surface trans-membrane protein, expressed mainly by the tumour cells. In this study we have estimated the urinary, serum and tissue levels of CD147 in patients known to be harbouring non-muscle invasive TCC of urinary bladder and compared them with controls. In the present study we investi- gated the levels of CD147 in serum and urine and cellular expression in the tu- mour tissue. Material and Methods: Thirty patients with non-muscle invasive urothelial carci- noma, two sets of controls: 15 patients with benign prostate hyperplasia and 15 healthy volunteers were included. Levels were estimated using ELISA kits in serum and urine. Total RNA was isolated from the tumour tissue sections of patients and normal urothelium of BPH patients, re- verse transcribed to cDNA and cellular expression was analysed by real-time PCR. Results: Significantly elevated levels of CD147 were observed in both serum and urine of TCC patients (108.5431.8pg/ml, 67.0824.98pg/ml) as compared to the controls (p0.008), with urinary levels showing significant correlation with grade of disease on comparison with both the controls (p0.001) and serum levels with healthy controls. The mRNA expression showed a 2.2 fold increase in patient as compared to the BPH controls. Conclusion: CD147 shows a significant increase in serum, urinary levels and cellu- lar expression in the patients with TCC, thus suggesting a possible role of this mol- ecule as a potential diagnostic marker for TCC. UP-01.035 Virtual Cystoscopy (Pneumo-Cystoscopy): Its Utility in the Prospective Evaluation of Bladder Tumor Singh I, Mehrotra G, Jaura M, Tandon A, Agarwal V, Joshi M University College of Medical Sciences (University of Delhi) and GTBH, New Delhi, India Introduction and Objectives: With the availability of three-dimensional computer- volume rendering techniques or virtual- reality imaging, it is now possible to per- form accurate intra-luminal imaging. Intra- luminal navigation through several hollow viscera like the colon, bronchus, stomach and urinary bladder has been reported in the literature. Urinary bladder may be an ideal intra-abdominal organ for performing virtual cystoscopy (VC) imaging with some studies comparing the relative accu- racy of VC and cystopanendoscopy. The literature regarding the accuracy of VC in detecting intravesical lesions is scarce and equivocal. This study aims to prospec- tively evaluate the utility of virtual cystos- copy versus cystopanendoscopy in the detection of bladder tumor(s). Materials and Methods: Ethical clear- ance was obtained from the Institutional ethics committee. After an informed con- sent thirty patients fulfilling the inclusion UNMODERATED POSTER SESSIONS UROLOGY 78 (Supplement 3A), September 2011 S195