Int.J.Curr.Microbiol.App.Sci (2020) 9(11): 978-982 978 Original Research Article https://doi.org/10.20546/ijcmas.2020.911.116 Histomorphological and Histochemical Study of Macula Densa in Guinea Fowl (Numida meleagris) Kidney Gajendra Singh*, Balwant Meshram and Hemant Joshi Department of Veterinary Anatomy and Histology, College of Veterinary and Animal Science, Navania, Udaipur, Rajasthan, India *Corresponding author ABSTRACT Introduction The Macula Densa, a specialized cellular structure of distal tubules which believed to be performed important sensory function that transmit chemical signals in the juxtaglomerular apparatus while controlling different vital kidney functions, includes renal blood flow, glomerular filtration, renin release and the changes in tubular fluid composition (Peti- Peterdi and Harris, 2010). The increase of sodium concentration in the tubular fluid leads to the decreased production of renin by extraglomerular mesangial cells and juxtaglomerular cells (Maynard and Downes, 2019). Each of the renal corpuscle has two either side located urinary pole and vascular pole. The area where renal corpuscle initiate different parts of uriniferous tubules which known as urinary pole, however, the vascular pole has different parts of Juxtaglomerular apparatus viz. afferent and efferent arterioles and macula densa cells which are situated generally in between afferent and efferent arterioles (Ogawa and Sokabe, 1971). The darker stained rounded or elongated basal nuclei are evident at apical position with euchromatin and the nucleolus (Cangiotti et al., 2018). International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 9 Number 11 (2020) Journal homepage: http://www.ijcmas.com The juxtaglomerular apparatus usually located at the vascular pole of nephrons, which comprises afferent and efferent arterioles along with a specialized form of cells known as macula densa. The elliptical-shaped macula densa originated from the convoluted part of distal tubule in a closely packed cellular mass. The macula densa is located at the exclusive spot stuck between afferent and efferent arterioles, wherein the salt concentration found with high variability. This macula densa believed to be performed as the renal sensor elements which could control many physiological functions like glomerular hemodynamic and renin secretion. Different staining procedures were employed while confirming different micro-morphological elements of macula densa. Different histoenzymic observations were also perceived during the attempt of present studies. Keywords Guinea fowl, Macula densa, Histomorphology, Histochemistry and Juxtaglomerular apparatus Accepted: 10 October 2020 Available Online: 10 November 2020 Article Info