ABSTRACTS SPECIAL SCIENTIFIC SESSION ABSTRACTS 5 July 2012 SPECIAL SCIENTIFIC SESSION IN ARABIC LANGUAGE SESSION OF THE INTERNATIONAL ASSOCIATION OF LAW AND FORENSIC SCIENCES SSS1-OP-01 Hepatotoxicity of benzene in petroleum pump workers and green tea protection Ashraf Emara 1 , Eman Draz 1 , Kaled Elmogassabi 2 1 Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine Tanta University Egypt 2 Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine Bani Gazi University Libya The current study investigated benzene hepatotoxicity and the role of green tea in reducing benzene hepatotoxic effect. The study was conducted on petroleum pump male workers. The subjects were divided into four groups each group contains 15 subject. Group 1 was benzene non exposed subject (negative control), group 11 was benzene non exposed subject with oral administration of green tea [900 mL (6 cups) tea/d in 150-mL (1-cup) amounts. Each 150 mL tea contained 0.5 g green tea (put in water after boiling)] for 6 months (positive control), group III was benzene exposed work- ers, and group IV was benzene exposed workers with administra- tion of green tea for six months. Benzene and its metabolites (phenol and trans-trans muconic acid) were measured in urine by UV spectrophotometry. Hepatototoxicity was investigated through- out analysis of serum enzymes activities of adenosine deaminase, 5´ nucleotidase, β-glucuronidase, arylsulfatase and liver enzymes; as- partate aminotransferase and alanine aminotransferase. Green tea oral administration in the positive control group shows no signifi- cant changes in all the studied parameters in comparison with the negative control group except ALT and phenol which show signif- icant increase and decrease respectively. Serum activities of all the studied enzymes were increased in benzene pump workers in com- parison with the control groups except ALT. All enzymes activities were reduced in benzene pump workers treated with green tea for six months in comparison with non treated benzene workers. the current study revealed that grean tea may reduced the hepatotoxic- ity that may be induced by occupational exposure to benzene. SSS1-OP-02 Evaluation of occupational exposure to radiation induced alteration in Macro-, micro- and trace elements and ultra-structural changes on hair in Egyptian radiographers Enas El Maddah 1 , Merfat Oreby 1 , Amany Mousa 2 , Rehab El Gharabawy 3 1 Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Tanta University, Tanta, Egypt 2 Department of Histology, Faculty of Medicine, Tanta University, Tanta, Egypt 3 Department of Pharmacology, Faculty of Pharmacy, Tanta University, Tanta, Egypt The purpose of this study is to provide documentation on the occupational exposure to radiation induced toxicity on hair in Egyptian radiographers. It was decided to investigate the macro-, micro- and trace element contents and ultra- structural changes resulting from exposure to radiation of scalp hair samples of radiographers. This study was carried out with 40 adult males and females (20–42 yr-of-age) from Tanta city, Egypt. Those subjects were divided into controls (n 0 20); persons with no known occupational exposure to radiation and radiographers (n 0 20). The controls were age- and lifestyle-matched to the radiog- raphers (n 0 20). Scalp hair samples were collected. Hair concentra- tion of chlorine (Cl), sodium (Na), calcium (Ca), iron (Fe), zinc (Zn), cupper (Cu), vanadium (V), cadmium (Cd), mercury (Hg), antimony (Sb), selenium (Se), manganese (Mn), terbium (Tb), barium (Ba) and chromium (Cr) were performed with inductively coupled plasma- optical emission spectrometer (ICP-OES). Hair samples were taken for electron microscopic analysis. The present study revealed that, Cl, Na, Ca, Fe, Zn, Cu, V, Cd and Hg concentrations were significantly increased and Sb, Se and Mn concentrations were significantly de- creased in both male and female radiographers. No significant changes were observed in Tb, Ba and Cr in both male and female radiographers. The majority of the elements had higher concentrations in female hair samples than in male hair samples. Electron microscop- ic analysis showed some changes in hair and these changes were more pounced in females and male radiographers. This Supplement was not sponsored by any outside commercial interests. Int J Legal Med (2012) 126 (Suppl 1):S23–S38 DOI 10.1007/s00414-012-0702-x