1 LBH is a cancer stem cell- and metastasis-promoting oncogene essential for WNT stem cell function in breast cancer Koteswararao Garikapati 1,2,7 , Kilan Ashad-Bishop 1,2,3,7 , Sunhwa Hong 1,2,7 , Rehana Qureshi 2,4,7 , Megan E. Rieger 2,7,9 , Linsey E. Lindley 2,7,10 , Bin Wang 2,7 , Diana J. Azzam 2,7,11 , Mahsa Khanlari 5,7,12 , Mehrad Nadji 5,7 , Chaitanya Jain 7 , Deukwoo Kwon 6,7 , Yuguang Ban 6,7 , Zhen Gao 6,7 , Steven X. Chen 6,7 , Andrew H. Sims 8 , Susan B. Kesmodel 1,2,7 , Joyce M. Slingerland 2,4,7,13 , and Karoline J. Briegel 1,2,7,14,* 1 Department of Surgery, Molecular Oncology Program 2 Braman Family Breast Cancer Institute, Sylvester Comprehensive Cancer Center 3 Sheila and David Fuente Graduate Program in Cancer Biology 4 Department of Medicine 5 Department of Pathology 6 Biostatistics and Bioinformatics Shared Resources, Sylvester Comprehensive Cancer Center, and Department of Public Health Sciences 7 University of Miami Miller School of Medicine, Miami, Florida, USA 8 Applied Bioinformatics of Cancer Research Group, University of Edinburgh Cancer Research Centre, MRC Institute of Genetics and Molecular Medicine, Edinburgh, UK 9 Present address: Thermo Fisher, Carlsbad, California, USA 10 Present address: Department of Dermatology, UT Southwestern Medical Center, Dallas, Texas, USA 11 Present address: Department of Environmental Health Sciences, Florida International University, Miami, Florida, USA 12 Present address: Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA 13 Present address: Department of Oncology, Georgetown University, Washington DC, USA 14 Lead Author * Correspondence: Karoline J. Briegel, Ph.D. DeWitt Daugherty Department of Surgery University of Miami Miller School of Medicine 1600 NW 10 th Avenue Miami, Florida 33136 Phone: 305-243-4770 E-mail: kbriegel@med.miami.edu Running Title: LBH promotes cancer stem cell abundance downstream of WNT Key Words: LBH; WNT; breast cancer; cancer stem cells; tumor progression; metastasis; chemoresistance; RNA-Seq; patient-derived tumors; mouse models (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint this version posted January 30, 2021. ; https://doi.org/10.1101/2021.01.29.428659 doi: bioRxiv preprint