Botanica Pacifca. A journal of plant science and conservaton. 2021. 10(1): 69–74 DOI: 10.17581/bp.2021.10102 69 ©Botanical Garden-Insttute FEB RAS. 2021 ABSTRACT We studied growth of potato plantlets of two cultivars under different constant polychromatic light-emitting diodes (LEDs) and dynamic lighting with the spect- rum modifcations during growth. Light sources constructed by us were sunbox SB, red-green-blue RGB, and full spectrum FS, red-blue. White luminescent lamps were used as control light. Dynamic lighting was achieved by transferring groups of plantlets from one box to another. Red/green/blue portions in the light sources were (in percents): 39R/39G/22B for SB, 63R/21G/16B for RGB, 74R/8G/18B for FS, and 22R/49G/29B for control. PPFD was set at 45 µmol/m 2 s. Morpho- metric measurements were made 14 and 28 days after planting. Plantlets developed differently from initial stages. Cultivar 'Red Scarlett' appeared to be more suscep- tible to different lighting than 'Innovator'. Light variations RGB and RGB-SB were the most favorable for plantlet development. This is the frst report on the dynamic lighting application for growing potato plantlets. Keywords: Solanum tuberosum, artifcial light, different spectra, light emitting diodes, in vitro, micropropagation, cultivar, cultivation РЕЗЮМЕ Наконена О.В., Сбботин Е.П., Гриенко О.В., Гаика И.В., Орловска И.Ю., Холин А.С., Голова Д.О., Сбботина Н.И., Бл- гаков В.П., Клин Ю.Н. Развитие микрорастений картоел in vitro под разлинми полиромнми СД спектрами и динамиеским освеением. М исследовали развитие микрорастений картоел дв сортов под разлинми постоннми полиромнми светодиоднми (СД) спектрами и динамиеским освеением с изменением спектра в про- ессе роста. Нами бли сконстрирован следие истоники освее- ни: солненй бокс SB, красно-зелено-синий RGB, и полнй спектр FS, красно-синий. В каестве контролного света исползовалис беле лми- несентне ламп. Динамиеское освеение достигалос птем переноса грпп микрорастений из одного бокса в дргой. Дол красного/зелено- го/синего спектров в истоника света бла (в проента): 39R/39G/22B дл SB, 63R/21G/16B дл RGB, 74R/8G/18B дл FS и 22R/49G/29B дл контрол. PPFD бл становлен на ровне 45 мкмол/м 2 с. Морометри- еские измерени проводили ерез 14 и 28 дней от наала ксперимента. Микрорастени развивалис по-разном с сам наалн стадий кспе- римента. Сорт 'Red Scarlett' оказалс более восприимивм к изменени освеени, ем 'Innovator'. Вариант освеени RGB и RGB-SB оказалис наиболее благопритнми дл развити микрорастеий картоел. Это пер- вое сообение о применении динамиеского освеени дл враивани микрорастений картоел. Клеве слова: Solanum tuberosum, картоел, исксственнй свет, разне спект- р, светодиод, микроклоналное размножение, in vitro, сорт, враивание Olga V. Nakonechnaya 1 * e-mail: markelova@biosoil.ru Evgeniy P. Subbotin 2 e-mail: s.e.p@list.ru Olga V. Grishchenko 1 e-mail: grishchenkoov@gmail.com Irina V. Gaftskaya 1 e-mail: gaftskaya@biosoil.ru Irina Yu. Orlovskaya 1 e-mail: irina-orlovskay@mail.ru Alexander S. Kholin 2 e-mail: a_kholin@ iacp.dvo.ru Dariya O. Goltsova 2 e-mail: gd89@list.ru Natalia I. Subbotina 2 e-mail: sale789@mail.ru Victor P. Bulgakov 1 e-mail: bulgakov@biosoil.ru Yuri N. Kulchin 2 e-mail: kulchin@iacp.dvo.ru 1 Federal Scientifc Center of the East Asia Terrestrial Biodiversity FEB RAS, Vladivostok, Russia 2 Institute of Automation and Control Processes FEB RAS, Vladivostok, Russia * corresponding author Manuscript received: 22.09.2020 Review completed: 04.12.2020 Accepted for publication: 08.12.2020 Published online: 11.12.2020 Olga V. Nakonechnaya 1 *, Evgeniy P. Subbotin 2 , Olga V. Grishchenko 1 , Irina V. Gaftskaya 1 , Irina Yu. Orlovskaya 1 , Alexander S. Kholin 2 , Dariya O. Goltsova 2 , Natalia I. Subbotina 2 , Victor P. Bulgakov 1 , Yuri N. Kulchin 2 In vitro potato plantlet development under different polychromatic LED spectra and dynamic illumination The development of technologies of all year-round cul- tivation of agricultural crops in protected conditions using artifcial illumination meets the challenges in the feld of food security. These technologies allow reducing risks asso- ciated with farming in open feld and receive stable harvest independently from the season. Potato, Solanum tuberosum L., is one of the most important crops cultivated all over the world. The use of seed material of a high quality can de- termine the abundance of harvest. The maximum yield can be achieved by using elite and super elite tubers, produced via method of apical meristems. The method helps to clean the seed material from viruses, and sanitated material can be easily multiplied using microclonal propagation method in vitro. The method helps to rapidly propagate valuable and rare plant cultivars (Anisimov & Chugunov 2014). Light is the one of critical factors affecting plant growth and developing. Growth, morphogenesis, and tube- rization of potato in vitro are affected by light. By changing (modifying) light parameters (spectrum, intensity, photo- period) researchers can control plant development in vitro (Seabrook 2005), and thus avoid the usage of plant growth regulators that may cause atypical conditions (Aksenova et