Pharmacogn J. 2018; 10(1):92-98 A Multifaceted Journal in the feld of Natural Products and Pharmacognosy www.phcogj.com | www.journalonweb.com/pj | www.phcog.net Original Article 92 Pharmacognosy Journal, Vol 10, Issue 1, Jan-Feb, 2018 INTRODUCTION Archidendron bubalinum (Jack) I.C Nielsen is an evergreen plant indigeneous to Tailand, Peninsular Malaysia and Sumatra in Indonesia, especially in Lampung province. Tis plant is belongs to Fabaceae or Leguminosae family, and also placed in Mimosaceae. According to Lim (2012), this species has many synonyms e.g Albizia acradena Miq., Cylindrokelupha bubalina (Jack) Kosterm, Feuilleea bubalina (Jack) Kuntze, Inga bubalina Jack, Ortholobium bubalinum (Jack) Kostern, Pithecellobium bigeminum (L.) Mart. var. bubalinum (Jack) Benth., Pithecellobium ellipticum sensu auct., Pithecellobium lobatum sensu Ridley. Te fruit of this plant has diferent local names such as julang-jaling (Lampung), jering utan (Riau), jering kabau (West Sumatra), kabau (Jambi, Palembang, Riau), kabeu (Bengkulu), kerdas or jering tupai (Malaysia), and nieng-no (Tailand). 1,2,3,4,5,6 Te strong pungent smelling fruits have an odor like jering (Archidendron jiringa) and petai (Parkia speciosa). Volatile Compound Analysis using GC-MS, Phytochemical Screening and Antioxidant Activities of the Husk of “Julang-Jaling” (Archidendron bubalinum (Jack) I.C Nielsen) from Lampung, Indonesia ABSTRACT Background: “Julang-jaling” (Archidendron bubalinum (Jack) I.C Nielsen) fruits are commonly used as traditional food and in the treatment of blood sugar and heart disease. However, the research about the phytochemicals of the parts of this plants and their bioactivity was rare. Objective: The recent study was aimed to analyze volatile compounds in the extract of “julang-jaling” husks and evaluate their antioxidant and antimicrobial activities. Material and methods: The extraction was conducted using n-hexane, ethyl acetate, and methanol as extracting agent. The phytochemical assay was performed for all extracts, and followed by volatile compound analysis using GC-MS. The antioxidant assay was performed using DPPH method, and the antimicrobial activity was conducted using agar disc diffusion method. Results: The phytochemical assay showed that all extracts of “julang-jaling” husks contain various phytoconstituents having potential bioactivity. All extracts exhibit antioxidant activity with different level of activity depend on the type of extract. The IC 50 value were 273.57 ppm, 324.913 ppm, 735 ppm, for ethyl acetate, methanol and n-hexane, respectively. All extracts were able to inhibit the growth of Bacillus cereus, with the highest antimicrobial activity was gained for ethyl acetate extract. Both ethyl acetate and methanolic extract have antimicrobial activity toward E. coli, but no extracts yield positive results for Aspergillus favus and Aspergillus niger. Conclusion: GC-MS analysis revealed the presence of hexadecanoic acids and their ester form in all extract, which might largely contribute in the antioxidant and antimicrobial activity. Key words: Archidendron bubalinum (Jack) I.C Nielsen, GC-MS, Phytochemicals, Antioxidant, Antimicrobial Activity. Candra Irawan 1 , Foliatini 1* , Hanaf 2 , Lilis Sulistiawaty 1 and Maman Sukiman 3 Candra Irawan 1 , Foliatini 1* , Hanaf 2 , Lilis Sulistiawaty 1 and Maman Sukiman 3 1 Department of Analytical Chemistry Polytechnic of AKA Bogor, Bogor 16158, INDONESIA. 2 Department of Food Industrial Quality Assurance Polytechnic of AKA Bogor, Bogor 16158, INDONESIA. 3 Department of Industrial Waste Treatment Polytechnic of AKA Bogor, Bogor 16158, INDONESIA. Correspondence Dr. Foliatini Department of Analytical Chemistry Polytechnic of AKA Bogor, Bogor 16158, INDONESIA. Phone no :+62-856-7653-523 Fax:+62-251-8650352 E-mail: foliatini@gmail.com History • Submission Date: 25-08-2017; • Review completed: 11-09-2017; • Accepted Date: 20-11-2017 DOI : 10.5530/pj.2018.1.17 Article Available online http://www.phcogj.com/v10/i1 Copyright © 2018 Phcog.Net. This is an open- access article distributed under the terms of the Creative Commons Attribution 4.0 International license. Cite this article: Irawan C, Foliatini, Hanaf, Sulistiawaty L, Sukiman M. Volatile Compound Analysis Using GC-MS, Phytochemical Screening and Antioxidant Activities of the Husk of “Julang-Jaling” (Archidendron bubalinum (Jack) I.C Nielsen) From Lampung, Indonesia. Pharmacog J. 2018;10(1):92-8. Te fruits have the favor of jering and are similarly used to favor food. Young seeds can be eaten raw in ulam or lalapan (traditional vegetable salad) or in chutney. People also used the fruits for treating blood sugar and heart disease. To our knowledge, the phytochemical composition, antioxidant and antimicrobial activity of the “julang-jaling” husks have not yet been found. Since the husks were potential as herbal medicine, the phytoconstituents of this husks and the relation between the chemicals and their bioactivity should be studied comprehensively. Mass spectrometry, coupled with chromatographic separations such as gas chromatography (GC/MS) is normally used for direct analysis of components existing in traditional medicines and medicinal plants. In recent years GC-MS studies have been increasingly applied for the analysis of medicinal plants as this technique has proved to be a valuable method for the analysis of